From 2007.igem.org

check for pOmpC + TATA_INSA by PCR

• lane 1: pOmpC + TATA_INSA (clone)
• lane 2: ligation mixture
• there is a band (around 550 bp) larger than 430 but less than 613

digestion for (pCinRHSL+OmpRBS)

• SpeI digestion O/N (overnight)
• Klenow fill the sticky end into blunt end (25 C, 20 min)
• PstI digestion
• DNA smeared (>_<)