NYMU Taipei/Lab Notes/2007 8 29

From 2007.igem.org

  • Plasmid extraction for [pOmpC+(RBS+EYFP)]
    • pOmpC+(RBS+EYFP) 1-1, 1-2, 2, 3, 4, 5 (duplicate the first tube for even balance)
    • Extract from 1.5ml LB medium to 20ul EB buffer
    • Stored in -20°C
  • EYFP spectral characteristics:
    • Excitation: 514nm
    • Emition: 527nm
  • Liquid culture for MGC clones
    • INS (human), IGFBP3 (human), IGFBP7 (human), IDE (mouse) for insulin project
    • PUM1 (human), AKAP1 (human) for m.t. project

Who attend?

  • 立翔