NYMU Taipei/Lab Notes/2007 9 10

From 2007.igem.org

  • Ligation A(H_INS_A)
    • H_INS_A1:2ul
    • pOmpC:2ul
    • 10X ligase:1 ul(YEA)
    • 10X buffer:1 ul(YEA)
    • ddH2O:4ul
    • total: 10ul
  • Ligation B(H_INS_A)
    • H_INS_A1:3ul
    • pOmpC:2ul
    • 10X ligase:1 ul (YEA)
    • 10X buffer:1 ul (YEA)
    • ddH2O:3ul
    • total: 10ul
  • ligation time:
  • Transformation
    • ligated DNA:2ul
    • compenent cell:100ul
  • apply the cells on the agar plate
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