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 New digestions were done with some modifications in the protocols used after some disadvantages during the second transformations. The procedures continued by doing ligation and assembling the first parts that are going to be sent to the IGEM-BioBrick, as our contribution to the IGEM Registry.
After difficult and pitfall situations and problems such as not having on time the necessary equipment, reagents, and materials which arrived late, we were able to achieve the construction and assembling of the parts toward construction of the devices. In order to achieve this objective, we have to work overnight everyday in the lab, including during the weekends.
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