From 2007.igem.org

PCR otsAF/otsAIR on otsA (413 bp, gp=A)
PCR otsAIF/otsAR on otsA (1077 bp, gp=B)

---

PCR otsAF/otsAR on A+B (1456 bp BglII/XhoI)
Digest pBca9145-Bca1144 (BglII/XhoI 2054+910, L)
Product pBca9145-BBa I716301

---

otsAF forward BglII site anneals to otsA cgtaaAGATCTATGAGTCGTTTAGTCGTAGTATC
otsAIF Removing BamHI site in otsA CGATGACATTATCTGGATtCACGATTATCACCTG
otsAIR Removing BamHI site in otsA CAGGTGATAATCGTGaATCCAGATAATGTCATCG
otsaR reverse XhoI site anneals to otsA cgtatCTCGAGttaGGATCCTTACGCAAGCTTTGGAAAGGTAGC