Restriction Enzyme
From 2007.igem.org
We have selected HindIII and BamHI for promoter insertion and BamHI and SacI for firefly luciferase and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner
| Enzyme | Source | Recognition Sequence | Cut |
|---|---|---|---|
| BamHI | Bacillus amyloliquefaciens |
5'GGATCC 3'CCTAGG |
5'---G GATCC---3' 3'---CCTAG G---5' |
| HindIII | Haemophilus influenzae |
5'AAGCTT 3'TTCGAA |
5'---A AGCTT---3' 3'---TTCGA A---5' |
| SacI | Streptomyces achromogenes |
5'GAGCTC 3'CTCGAG |
5'---GAGCT C---3' 3'---C TCGAG---5' |
