Restriction Enzyme

From 2007.igem.org

We have selected HindIII and BamHI for promoter insertion and BamHI and SacI for firefly luciferase and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner

EnzymeSourceRecognition SequenceCut
BamHIBacillus amyloliquefaciens
5'GGATCC
3'CCTAGG
5'---G     GATCC---3'
3'---CCTAG     G---5'
HindIIIHaemophilus influenzae
5'AAGCTT
3'TTCGAA
5'---A     AGCTT---3'
3'---TTCGA     A---5'
SacIStreptomyces achromogenes
5'GAGCTC
3'CTCGAG
5'---GAGCT  C---3'
3'---C  TCGAG---5'