Sample | Date | Plasmid | Clone | Primer | Result | File Link
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KD001 | 6/12/07 | pBca9145-I716153 | hemB2 | ca998 | Ok, maybe short | KD001
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KD002 | 6/12/07 | pBca9145-I716153 | hemB4 | ca998 | Good, need to reverse sequence to get last 400bp | KD002
|
KD003 | 6/12/07 | pBca9145-I716155 | hemD2 | ca998 | Too short | KD003
|
KD004 | 6/12/07 | pBca9145-I716155 | hemD4 | ca998 | Good, need to reverse sequence to get last 200bp | KD004
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KD005 | 6/13/07 | pBca9145-I716153 | hemB4 | G101001 | Good, after reverse complementing, the first 100bp are off, but this part of the sequence is confirmed by KD002 | KD005
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KD006 | 6/13/07 | pBca9145-I716155 | hemD4 | G101001 | Good, after reverse complementing, the first 100bp are off, but this part of the sequence is confirmed by KD004 | KD006
|
KD007 | 6/14/07 | pBca9145-I716151 | hemA3 | ca998 | Not good, lots of mutations | KD007
|
KD008 | 6/14/07 | pBca9145-I716151 | hemA3 | G01001 | Not good, lots of mutations | KD008
|
KD009 | 6/14/07 | pBca9145-I716154 | hemC1 | ca998 | Good | KD009
|
KD010 | 6/14/07 | pBca9145-I716154 | hemC1 | G01001 | Good | KD010
|
KD011 | 6/15/07 | pBca9145-I716151 | hemA2 | ca998 | GC-->CG, but in both hemA3 and hemA2 clones. Chris says we cloned a different template from one in pubmed and to proceed | KD011
|
KD012 | 6/15/07 | pBca9145-I716151 | hemA2 | G01001 | Good | KD012
|
KD013 | 6/15/07 | pBca9145-I716154 | hemC2 | ca998 | Good, need reverse sequence | KD013
|
KD014 | 6/15/07 | pBca9145-I716154 | hemC2 | G01001 | Good | KD014
|
KD015 | 6/20/07 | pBca9145-I716152 | hemAC1 | ca998 | Not good, some mutations | KD015
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KD016 | 20|07 | pBca9145-I716152 | hemAC1 | G01001 | Ok, but forward has mutations | KD016
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KD017 | 6/20/07 | pBca9145-I716152 | hemAC2 | ca998 | Good, need reverse sequence | KD017
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KD018 | 6/20/07 | pBca9145-I716152 | hemAC2 | ca998 | Good, need reverse sequence | KD018
|
KD019 | 6/21/07 | pBca9145-I716152 | hemAC2 | G01001 | Good | KD019
|
KD020 | 6/26/07 | pBca9145-I716161 | 1106A+hemA1 | ca998 | Good | KD020
|
KD021 | 6/26/07 | pBca9145-I716162 | 1106A+hemAC2 | ca998 | Good | KD021
|
KD022 | 6/26/07 | pBca9145-I716163 | 1106A+hemB2 | ca998 | Good | KD022
|
KD023 | 6/26/07 | pBca9145-I716164 | 1106A+hemC2 | ca998 | No sequence, possibly problem with miniprep | KD023
|
KD024 | 6/26/07 | pBca9145-I716165 | 1106A+hemD2 | ca998 | Good | KD024
|
KD025 | 7/5/07 | pBca9145-I716167 | ShemAC+hemB1 | ca998 | Good | KD025
|