Tokyo/suggestions
From 2007.igem.org
Suggestion 1.
Merit:
The repressor R1 can be inserted after constructing the other part.
Suggestion 2.
Merit:
A ribosome binding site here is easily exchangeable; therefore, the expression level can be modulated without changing the promoter and regulators.
Suggestion 3.
Merit:
Promoters can be easily exchanged, and the expression level can be modulated at promoter level.
Suggestion 4.
Merit:
The precursor of this part was constructed for the functional check of BioBrick parts and available;
therefore, this construction can be done in much shorter time.