From 2007.igem.org

Overnight (O/N) Preparation:

1. Put 2.5 mL of LB Broth into a 15 mL tube. (Make sure to flame the broth)
2. Scrape off a single colony from a plate with a pipette tip and eject tip into the tube.
3. Add 2.5 μL of the appropriate antibiotics. (Check spreadsheet)
4. Incubate overnight.

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