1. To 50uL competent cells add 1uL DNA solution

2. Incubate on ice for 45min

3. Heat shock at 42 degrees for 1min

4. Incubate on ice for 15min

5. Add 1ml LB

6. Incubate at 37 degrees for 1 hour

7. Pellet Cells

8. Pour off majority of LB

9. Resuspend cells in remaining LB

10.Plate resuspended cells and incubate overnight at 37 degrees