From 2007.igem.org

2- Yeast Strains

Saccharomyces cerevisiae strains: (1) SP163 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-RR, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2), (2) SP164 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-HMTM1, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2),(3) SP165 (W303 MATa, bar1::NatR, far1D, ste2D, ho:: pSte2-Ste2-GFP-WMTM1, mfa2::pCyc-mCherry-PH-ING2, his 3, trp1, leu2).

Subsequently, each pRS315-based construct was transformed (selection based on Leu2 marker) into SP163.