# USTC/NamingRules

### From 2007.igem.org

Biologists always use symmetric LacI-family operators, e.g. AATTGTGAAC GTTCACAATT (O44) and AATTGTAAGC GCTTACAATT (O22). Please notice that they are all 20bp long and each right half of 10bp-long strandâ€™s complementary strand is reversed symmetric with its left half of 10bp-long strand. For short we call this kinds of symmetrical operators as Oxx, for example, O44, O22, etc. Then you must have already understood what O24 means-- Yes, it means the asymmetric operator AATTGTAAGC GTTCACAATT (O24), which has the 10bp-long strand of O22 for its left half sequence and the 10bp-long one of O44 for its right half sequence. Again for example, O42 means AATTGTGAAC GCTTACAATT (O42), and so on. We also give these asymmetric operators another name: Hybrid Operators.

For convenience, I listed the sequences we've used:

### Symmetric Operators

O11 AATTGTGAGC GCTCACAATT O22 AATTGTAAGC GCTTACAATT O33 AATTGTAAAC GTTTACAATT O44 AATTGTGAAC GTTCACAATT O55 AATTTTGAGC GCTCAAAATT O66 AATTATGAGC GCTCATAATT O77 GACGACTGTA TACAGTCGTC

### Asymmetric Operators

**"Ox6", "Ox7" and "O6x"**

O16 AATTGTGAGC GCTCATAATT O26 AATTGTAAGC GCTCATAATT O46 AATTGTGAAC GCTCATAATT O61 AATTATGAGC GCTCACAATT O17 AATTGTGAGC TACAGTCGTC O27 AATTGTAAGC TACAGTCGTC O47 AATTGTGAAC TACAGTCGTC

**"Oxy"**

O12 AATTGTGAGC GCTTACAATT O21 AATTGTAAGC GCTCACAATT O24 AATTGTAAGC GTTCACAATT

### Those Operators

### Loaded on Double Report System