Week 10

From 2007.igem.org

09/03/07
  • Miniprep for:

-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S0100 S0100];

-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S03520 S03520];

-[http://partsregistry.org/Part:BBa_I763005 I763005]+[http://partsregistry.org/Part:BBa_I763015 I763015];

-[http://partsregistry.org/Part:BBa_J52034 J52034]+[http://partsregistry.org/Part:BBa_E0022 E0022];

  • Digestion for:

-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S0100 S0100] with Eco/Spe;

-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S03520 S03520] with Eco/Spe;

-[http://partsregistry.org/Part:BBa_I763005 I763005]+[http://partsregistry.org/Part:BBa_I763015 I763015] with Spe/Pst1;

-[http://partsregistry.org/Part:BBa_J52034 J52034]+[http://partsregistry.org/Part:BBa_E0022 E0022] with Spe/Pst1;

-[http://partsregistry.org/Part:BBa_B0015 B0015] with Eco/Xba;

  • Band extraction from gel for all digestion;
  • Ligation for:

-[http://partsregistry.org/Part:BBa_I763025 I763025]+[http://partsregistry.org/Part:BBa_B0015 B0015]([http://partsregistry.org/Part:BBa_I763026 I763026]);

-[http://partsregistry.org/Part:BBa_I763024 I763024] +[http://partsregistry.org/Part:BBa_B0015 B0015]([http://partsregistry.org/Part:BBa_I763030 I763030]);

-[http://partsregistry.org/Part:BBa_I763016 I763016]+[http://partsregistry.org/Part:BBa_I763020 I763020]([http://partsregistry.org/Part:BBa_I763019 I763019]);

-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_P0412 P0412]([http://partsregistry.org/Part:BBa_I763021 I763021]);

-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_S0100 S0100]([http://partsregistry.org/Part:BBa_I763023 I763023]);

-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_S03520 S03520]([http://partsregistry.org/Part:BBa_I763022 I763022]);

-[http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_I763020 I763020]([http://partsregistry.org/Part:BBa_I763011 I763011]);


09/04/07
  • There are colonies for every ligations.
  • Meeting.


09/05/07

Miniprep for:

- [http://partsregistry.org/Part:BBa_I763016 I763016]+ [http://partsregistry.org/Part:BBa_I763020 I763020] ([http://partsregistry.org/Part:BBa_I763019 I763019]);

- [http://partsregistry.org/Part:BBa_I763025 I763025]+ [http://partsregistry.org/Part:BBa_B0015 B0015] ([http://partsregistry.org/Part:BBa_I763026 I763026]);

- [http://partsregistry.org/Part:BBa_I763024 I763024]+ [http://partsregistry.org/Part:BBa_B0015 B0015] ([http://partsregistry.org/Part:BBa_I763030 I763030]);

- [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I763020 I763020] ([http://partsregistry.org/Part:BBa_I763011 I763011]);

- [http://partsregistry.org/Part:BBa_I763009 I763009]+ [http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763021 I763021]);

- [http://partsregistry.org/Part:BBa_I763009 I763009]+ [http://partsregistry.org/Part:BBa_S03520 S03520]([http://partsregistry.org/Part:BBa_I763022 I763022]);

  • Digestion for:

- [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba;

- [http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe;

- [http://partsregistry.org/Part:BBa_I763030 I763030] with Eco/Spe;

- [http://partsregistry.org/Part:BBa_I763011 I763011] with Xba/Pst1;

- [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I13507 I13507]([http://partsregistry.org/Part:BBa_I763007 I763007]) with Xba/Pst1;

- [http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe;

  • Control digestion for [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Pst1;
  • Band extraction from gel for:

- [http://partsregistry.org/Part:BBa_I763019 I763019];

- [http://partsregistry.org/Part:BBa_I763026 I763026];

- [http://partsregistry.org/Part:BBa_I763011 I763011];

- [http://partsregistry.org/Part:BBa_R0051 R0051]+ [http://partsregistry.org/Part:BBa_I13507 I13507] ([http://partsregistry.org/Part:BBa_I763007 I763007]);

  • New control digestion for:

-[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Spe;

-[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Pst1;

-[http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe;

-[http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe;

-[http://partsregistry.org/Part:BBa_I763030 I763030] with Eco/Spe;

  • Band extraction for [http://partsregistry.org/Part:BBa_I763019 I763019] (digested with Xba/Spe and with Xba/Pst1), [http://partsregistry.org/Part:BBa_I763026 I763026];
  • Ligation O/N for:

-[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763019 I763019]([http://partsregistry.org/Part:BBa_I763028 I763028]);

-[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763004 I763004]([http://partsregistry.org/Part:BBa_I763027 I763027]);


09/06/07

Digestion for:

-[http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba;

-[http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe;

-[http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe.

  • Band extraction from electrophoresis gel for [http://partsregistry.org/Part:BBa_I763019 I763019] and [http://partsregistry.org/Part:BBa_I763026 I763026].
  • Ligation for [http://partsregistry.org/Part:BBa_I763027 I763027] with new extracts.
  • Transformations for the new ligations.


09/07/07
  • Glycerol stocks preparation;
  • We check without plasmid bacteria growth curve to individuate a model parameter.


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