From 2007.igem.org

10/15/07

• O/N ligations for:

-I763019;

-I763039.

10/16/07

• Transformation of I763019 and I763039.
• We inoculate:

-I763005;

-I763015;

-I763004.

• Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.

10/17/07

• Miniprep of:

-I763005;

-I763015;

-I763004.

• We inoculate:

-I763029;

-I763039 (high copy).

• Control Digestion of the following Minipreps for results impagination:

-I763020;

-I763004;

-I763011;;

-I763007 (on high copy plasmid);

-I763007 (on low copy plasmid);

-I763033;

-I763015;

-I763012;

-I763005;

-I763031.

• Gel electrophoresis of the fragments above.

10/18/07

• Miniprep:

-I763029;

-I763039 (high copy).

• Digestion for:

-I763029 (Eco/Pst1)

• Band extraction of:

-I763029 (Eco/Pst1)

• Ligation for:

-pSB4A3 (Eco/Pst1)

-I763029 (Eco/Pst1)

• Ligation for:

-I763004 (Xba/Pst1)

-I763026 (Spe/Pst1)

• Control Digestion of the following Minipreps for results impagination:

- I763016 (low copy plasmid);

- I763016 (high copy plasmid);

- I763036;

- I763019;

- I763032;

- I763025 (low copy plasmid);

- I763025 (high copy plasmid);

- I763026;

- I763035.

• Gel electrophoresis of the fragments above.

10/19/07

• Transformation:

-I763034 (on high copy plasmid);

-I763039;

-I763029 on pSB4A3 (low copy plasmid).

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