Wisconsin/Protocol:Digestion

From 2007.igem.org

Endonuclease Digestion

  • 10uL ddH2O
  • 5uL DNA
  • 2uL 10x buffer
  • 2uL 1:10 dilution of BSA soln
  • 0.5uL Enzyme 1
  • 0.5uL Enzyme 2

Incubate at 37C for 2-4 hours

Use [http://www.promega.com/guides/re_guide/RESearch.asp this website] for restriction enzyme and [http://www.promega.com/guides/re_guide/RESearch.asp?search=buffer this site] for checking buffer compatibility.

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