From 2007.igem.org

Primer Stock

First check primer concentration using spectrophotometer... then prepare stock of 500uL 10uM.

Basic Non-Sequencing PCR

• 38.5uL H₂O
• 5uL 10x ExTaq buffer
• 1uL 10mM dNTPs
• 1uL Forward Primer
• 1uL Reverse Primer
• 1uL Template
• 0.5uL ExTaq (add this last)
• 2uL MgCl₂ 25mM

Run on Ansari PCR machine CDC-02

PCR Purification

Follow instruction in the [http://www1.qiagen.com/literature/protocols/QIAquickSpin.aspx QIAquick Spin Handbook]