Bologna University/M9 medium

'''Preparation instructions:'''
::1. Dissolve 56.4g in 1L of distilled water.
::2. Autoclave for 15min at 121°C.
This convenient 5x concentrate can be stored and diluted as needed to prepare 5L of 1x M9 minimal salts.
For M9 minimal medium:
::1. Aseptically dilute 200mL of M9 minimal salts, 5x [ ] with 800mL of sterile water, if necessary, cool to 45-50°C.
::2. Aseptically add 20mL of sterile 1M glucose and 2mL of sterile 1M magnesium solfate to prepare 1L of M9 minimal medium.
::3. If desired aseptically add 0.1mL of 1M sterile calcium chloride to the M9 minimal medium. M9 minimal medium may also be supplemented with the appropriate amino acids.

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