Chiba/Weekly

== Week1(8/16~8/23) ==
'''Monday,August,17,2007'''
*pUC-FliCのPCR
{| border="1" cellspacing="0" cellpadding="4" align="center"
|-
!F/R
!FliC-207
!FliC-228
!FliC-240
!FliC-252
!FliC-267
|-
|FliC-207
|①
|
|
|
|
|-
|FliC-228
|②
|③
|
|
|
|-
|FliC-240
|④
|⑤
|⑥
|
|
|-
|FliC-252
|⑦
|⑧
|⑨
|⑩
|
|-
|FliC-267
|⑪
|⑫
|⑬ 
|⑭
|⑮
|-
|}



{| border="1" cellspacing="0" cellpadding="4" align="center"
|-
!Recipe(単位：μ)
!①
!②
!③
!④
!⑤
!⑥
!⑦
!⑧
!⑨
!⑩
!⑪
!⑫
!⑬
!⑭
!⑮
|-
|pUC19-FliC
|1
|1
|1
|1
|1
|1
|1
|1
|1
|1
|1
|1
|1
|1
|1
|-
|FliC-207F
|5
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|-
|FliC-207R
|5
|5
|
|5
|
|
|5
|
|
|
|5
|
|
|
|
|-
|FliC-228F
|
|5
|5
|
|
|
|
|
|
|
|
|
|
|
|
|-
|FliC-228R
|
|
|5
|
|5
|
|
|5
|
|
|
|5
|
|
|
|-
|FliC-240F
|
|
|
|5
|5
|5
|
|
|
|
|
|
|
|
|
|-
|FliC-240R
|
|
|
|
|
|5
|
|
|
|5
|
|
|5
|
|
|-
|FliC-252F
|
|
|
|
|
|
|5
|5
|5
|5
|
|
|
|
|
|-
|FliC-252R
|
|
|
|
|
|
|
|
|
|5
|
|
|
|5
|
|-
|FliC-267F
|
|
|
|
|
|
|
|
|
|
|5
|5
|5
|5
|5
|-
|FliC-267R
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|5
|-
|10×Buffer
|10
|10
|10
|10
|10
|10
|10
|10
|10
|10
|10
|10
|10
|10
|10
|-
|dNTPmix
|10
|10
|10
|10
|10
|10
|10
|10
|10
|10
|10
|10
|10
|10
|10
|-
|Vent
|1
|1
|1
|1
|1
|1
|1
|1
|1
|1
|1
|1
|1
|1
|1
|-
|dH2O
|68
|68
|68
|68
|68
|68
|68
|68
|68
|68
|68
|68
|68
|68
|68
|-
|Total
|100
|100
|100
|100
|100
|100
|100
|100
|100
|100
|100
|100
|100
|100
|100
|-
|}

== Week2(8/24~8/31) ==


== Week3(9/1~9/8) ==


== Week4(9/9~9/15) ==
*Swaming test
#FliC-267His-RBSプラスミドをJW1908(KEIO⊿FliC)にTransformation（Positive Control=ASKA,Negative Control=KEIO-JW1908にFliC-267HisをTransformしたもの）
#LB培地で37℃12h2ml培養
#トリプトン培地（Agar0.25%）の中央に1μinoculate
#30℃、24-48h,Taxis
#Taxis ling を観察
*'''Sample'''

[[Image:swam4-2-2.jpg]]

== Week5(9/16~9/22) ==


== Week6(9/23~9/29) ==


== Week7(9/30~10/6) ==