Melbourne/Secondary Reagent NYZ

[[Melbourne/Protocols for Secondary Reagents|<return to list of secondary methods>]]  [[Melbourne|  <team home page>]]
===Preparation of NZY media===

===Uses===
*Site-directed mutagenesis

===Ingedients:===
Per Liter:

*10g [[Melbourne/primary NZ amine|NZ amine]]
*5g [[Melbourne/primary Nacl|NaCl]]
*5g [[Melbourne/primary Bacto-yeast Extract|Bacto-yeast Extract]]
*[[Melbourne/primary NaOH|NaOH]] to adjust pH
*Sterile water

===Method:===
#Mix ingredients in Sterile water using a magnetic stirrer
#Adjust pH to 7.5 using NaOH.

===Equipment:===
Magnetic Stirrer