NYMU Taipei/Protocols2/Literature reiew

<h3>Engineering Escherichia coli to see light</h3>
<p>&nbsp;Levskaya et al., Nature, Volume 438, Pages 441-442, 2005</p>
<p>To create the chimaera, we aligned members of the phytochrome family with EnvZ and identified potential functional crossover points between the <em>Synechocystis</em> phytochrome Cph1 and EnvZ. The length and composition of the peptide that links a photoreceptor to its response-regulator can affect signal transduction, and we therefore constructed a series of chimaeras with variable linker lengths. The variants were transformed into a <img alt="Delta" align="bottom" border="0" src="http://www.nature.com/__chars/_delta/black/med/base/glyph.gif" />EnvZ <em>E. coli</em> strain containing a chromosomal fusion between the OmpR-dependent <em>ompC</em> promoter and the <em>lacZ</em> reporter, which enzymatically produces a black compound.</p>
<ol>
    <li>The active chimera contains the first 517 a.a. of Cph1 and he last 229 a.a. of EnvZ</li>
</ol>
<p>&nbsp;</p>
<hr width="100%" size="2" />
<h3>&nbsp;Mutational Analysis of the Cytoplasmic Linker Region of Taz1-1, a Tar-EnvZ Chimeric Receptor in <em>Escherichia coli</em></h3>
<p>&nbsp;</p>
<p>Tian Jin and Masayori Inouye, <a href="http://www.sciencedirect.com/science/journal/00222836">Journal of Molecular Biology</a>, <a href="http://www.sciencedirect.com/science?_ob=PublicationURL&amp;_tockey=%23TOC%236899%231994%23997559994%23309289%23FLT%23&amp;_cdi=6899&amp;_pubType=J&amp;view=c&amp;_auth=y&amp;_acct=C000053839&amp;_version=1&amp;_urlVersion=0&amp;_userid=1576506&amp;md5=6c6e3768d1200349e03939accd754f65">Vol 244</a>, Pages 477-481, 1994</p>
<ol>
    <li>Most receptors in E. coli located in the cytoplasmic membrane and share similar transmembrane topology.</li>
    <li>The N-terminal extracytoplasmic domains are flanked by two transmembrane domains.</li>
    <li>The second transmembrane domain is connected to the cytoplasmic region designated as a &quot;linker&quot;, which is followed by the cytoplasmic signaling domain.</li>
    <li>&nbsp;</li>
</ol>
<p>&nbsp;</p>
<hr width="100%" size="2" />
<p>&nbsp;</p>