Virginia Tech/Updates/Plasmid

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<center><h1><font color="#ff8c00"> Reporter Plasmid Updates:
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<center><h3><font color="#8b0000"> VT iGEM Project 2007: Engineering an Epidemic</font></h3></center>
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<p><h3>Introduction: The Reporter Plasmid</h3>
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In order to collect data for our experiments, we needed a way to tell whether an infected cell was lysogenic or lytic. We decided that a good way to do this would be to use a reporter plasmid containing the lambda promoter and two fluorescent genes. The same chemicals that decided which pathway the cell entered would decide which fluorescent protein from our reporter plasmid to express.</p>
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[[Image:Lambda_Pr_rep.JPG]]
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<p><h3>Updates</h3></p>
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<p>'''Designing the Promoter Construct'''<br>
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''July 24, 2007''</p>
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<p>Click [[ here]] to read about how we designed the reporter plasmid's main feature, the promoter construct.</p>
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<html><center><A href="https://2007.igem.org/Virginia_Tech/Updates" target=_top><h3><FONT color=#ff8c00>Return to Progress Updates</FONT></h3></A></center></html>
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Revision as of 17:08, 24 July 2007

Home

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Reporter Plasmid Updates:

VT iGEM Project 2007: Engineering an Epidemic

Introduction: The Reporter Plasmid

In order to collect data for our experiments, we needed a way to tell whether an infected cell was lysogenic or lytic. We decided that a good way to do this would be to use a reporter plasmid containing the lambda promoter and two fluorescent genes. The same chemicals that decided which pathway the cell entered would decide which fluorescent protein from our reporter plasmid to express.

Lambda Pr rep.JPG

Updates

Designing the Promoter Construct
July 24, 2007

Click here to read about how we designed the reporter plasmid's main feature, the promoter construct.


Return to Progress Updates


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