Template:BerkiGEM2007 HannahConstructionFiles

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[[I716316]]
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[[BerkiGEM2007-HannahConstructionFile1 | Construction of I716316)]]<br>
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PCR HNX-F and HNX-R on H-Nox  (567bp, BglII/XhoI)
 
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Sub into pBca1144              ( BglII/XhoI, 2054+910  L)
 
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Product is pBca1144-H-NOX
 
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HNX-F Forward primer anneals to BglII ctagtAGATCTATGAAGGGGACAATCGTCGG
 
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HNX-R Reverse primer anneals to XhoI cgtgaCTCGAGgaatgGGATCCTTAATTTTTCTTATACTCA
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[[I716319]]
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[[I716320]]

Latest revision as of 22:27, 24 July 2007

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