Melbourne/meeting 27 July 2007

From 2007.igem.org

(Difference between revisions)
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= 5:30 Action points from last meeting:=
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== 5:30 Action points from last meeting:==
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* Alisa: Wiki "Parts_used status" up to date? -(outstanfding from 19/7/07)
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* <b>Alisa: </b>Wiki "Parts_used status" up to date? -(outstanfding from 19/7/07)
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* Jan: Lecture Timetables spread sheet complete: attached.
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* <b>Jan:</b> Lecture Timetables spread sheet complete: attached.
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* Phil: Status of #BBa-M30109 & #BBa-V1012 = "will ship this week".
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* <b>Phil:</b> Status of #BBa-M30109 & #BBa-V1012 = "will ship this week".
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* Jan: Production of sequence for blue light receptor? " -designed
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* <b>Jan:</b> Production of sequence for blue light receptor? " -designed
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= 5:45 Status Reports: (5 minutes each including any discussion)=
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== 5:45 Status Reports: (5 minutes each including any discussion)==
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* Alisa: Ligation experiments progress.
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* <b>Alisa: </b> Ligation experiments progress.
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**
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** Problem: 1.2KB genes between biobrick forward & reverse primers not being visualised in colony PCR with 1.5min extn time. (300bp fragments do amplify)
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* Alisa: Red light receptor progress/design/plan- needs.
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*** - <b>craig</b> to try and repeat.
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** Plan to be completed to be put on wiki this week
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*** - <b>Alisa: </b> has digestion in progress as alternative diagnostic to prove if problem is ligation or PCR.
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* Jan: Blue light receptor design/plan - needs.
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*** - <b>Alisa: </b>believes alkaline phosphatase damage is cause of problem.
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** design completed, Plan to be completed and put on wiki this week
+
* <b>Alisa: </b> Red light receptor progress/design/plan- needs.
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* Phil: Gas vesicles progress/design/plan - needs.
+
** Plan to be completed to be put on wiki this week, estimated at six weeks duration.
 +
* <b>Jan:</b> Blue light receptor design/plan - needs.
 +
** design completed, Plan to be completed and put on wiki this week -<b>Jan</b>
 +
** Theory to be put on the wiki this week -<b> Jan</b>,-<b>Alisa </b> to review
 +
** ComA to be ordered by tuesday under discount plan
 +
** ComP fusion to be ordered ASAP.
 +
 +
* <b>Phil:</b> Gas vesicles progress/design/plan - needs.
** Plan up to basic biobrick complete,  
** Plan up to basic biobrick complete,  
** Primers not requested for Friday delivery so cant proceed this week end.
** Primers not requested for Friday delivery so cant proceed this week end.
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** Biobrick prefix/sufix primers to be designed this week.
** Biobrick prefix/sufix primers to be designed this week.
** Plan for support parts/test harness required.
** Plan for support parts/test harness required.
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* Kasia: Documentation progress vs last report/ new problems/ needs.-No report
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** Some items require ordering,round bottom falcon tubes, or manufacture NZY broth, blue/white plate.
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=6.15 New Agenda Items:=
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** Lac promotor and antibiotic death plasmids required.
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* Phil: Individuals desired time commitment?
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*      <b>Pat:</b>
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eg 12hrs per week equiv to an extra subject,
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** Will transform, growup and prepare glycerol stocks of death plasmid in DB3.1 this week.
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or 3 hrs on wed afternoon only...
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* <b>Kasia:</b> Documentation progress vs last report/ new problems/ needs.-No report
-
+
==6.15 New Agenda Items:==
 +
* <b>Phil:</b> Individuals desired time commitment:
 +
{|border="1"
 +
!Individual!!Desired time committment
 +
|-
 +
|Pat|| About 4h/wk Monday afternoon or Tuesday morning
 +
|-
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|Alisa|| About 4h/wk - Thursday afternoon
 +
|-
 +
|Jan || About 10h/pw - flexible weekday mornings
 +
|-
 +
|Phil || About 18h/pw - Thursday morning, Friday, Saturday or Sunday,
 +
|}
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* Phil: Administrative Timetable: (12 Weeks to go)
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* <b>Phil:</b> Administrative Timetable: (12 Weeks to go)
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Fab request & project description by Tuesday 31 July.
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** Fab request & project description by Tuesday 31 July.
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"Produce and characterize buoyant bacteria  
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** "Produce and characterize buoyant bacteria and light receptor components of coliforming system."
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and light receptor components of coliforming system."
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* <b>Next meeting: <b/> 6th August (Monday) 1.15PM agreed but latter changed to 3rd Aug (Friday) 5:30PM.
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=6:30 End =
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==6:30 End ==
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=Igem Administrative Timetable:=
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==Igem Administrative Timetable:==
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*31 July Must lodge fabrication request to receive quota.
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{|border="1"
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*1 August Team project descriptions due.  
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|-
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*1 September Final team rosters due.   
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|1 August || Team project descriptions due.  
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*1 September Jamboree attendance fees due  $100USD per team
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|-
 +
|1 September || Final team rosters due.   
 +
|-
 +
|1 September|| Jamboree attendance fees due  $100USD per team
member.  
member.  
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*26 October  Project and part documentation due DNA received USA.
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|-
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*26 October BioBrick Part DNA received by the Registry.   
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|26 October|| Project and part documentation due DNA received USA.
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*3-4 November iGEM Competition Jamboree, MIT, USA.   
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|-
-
 
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|26 October || BioBrick Part DNA received by the Registry.   
-
 
+
|-
 +
|3-4 November || iGEM Competition Jamboree, MIT, USA.   
 +
|}
.
.
-
** Some items require ordering,round bottom falcon tubes, or manufacture NZY broth, blue/white plate.
 
-
** Lac promotor and antibiotic death plasmids required.
 
-
** Primers on critical path    -------phil to complete ASAP.
 
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*Jan Status report on Blue light sensor and light box apperatus, lead time on DNA fabrication?
 
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** Plan to have biobrick fabricated (up to 3Kb on fast turnarround= 525USD.)
 
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*Adhesion: to be abbandoned for this year, unless clever idea comes to mind.
 
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* Documentation wiki status:
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== Documentation wiki status:==
**Priority should be on plans and experimental report analysis and conclusions-----Alisa .
**Priority should be on plans and experimental report analysis and conclusions-----Alisa .
**If only Pat & Alisa doing lab work not much documentation needed.
**If only Pat & Alisa doing lab work not much documentation needed.

Revision as of 03:11, 2 August 2007

Contents

5:30 Action points from last meeting:

  • Alisa: Wiki "Parts_used status" up to date? -(outstanfding from 19/7/07)
  • Jan: Lecture Timetables spread sheet complete: attached.
  • Phil: Status of #BBa-M30109 & #BBa-V1012 = "will ship this week".
  • Jan: Production of sequence for blue light receptor? " -designed

5:45 Status Reports: (5 minutes each including any discussion)

  • Alisa: Ligation experiments progress.
    • Problem: 1.2KB genes between biobrick forward & reverse primers not being visualised in colony PCR with 1.5min extn time. (300bp fragments do amplify)
      • - craig to try and repeat.
      • - Alisa: has digestion in progress as alternative diagnostic to prove if problem is ligation or PCR.
      • - Alisa: believes alkaline phosphatase damage is cause of problem.
  • Alisa: Red light receptor progress/design/plan- needs.
    • Plan to be completed to be put on wiki this week, estimated at six weeks duration.
  • Jan: Blue light receptor design/plan - needs.
    • design completed, Plan to be completed and put on wiki this week -Jan
    • Theory to be put on the wiki this week - Jan,-Alisa to review
    • ComA to be ordered by tuesday under discount plan
    • ComP fusion to be ordered ASAP.
  • Phil: Gas vesicles progress/design/plan - needs.
    • Plan up to basic biobrick complete,
    • Primers not requested for Friday delivery so cant proceed this week end.
    • Mutagenesis primers will be redesigned by hand for delivery next Friday.
    • Biobrick prefix/sufix primers to be designed this week.
    • Plan for support parts/test harness required.
    • Some items require ordering,round bottom falcon tubes, or manufacture NZY broth, blue/white plate.
    • Lac promotor and antibiotic death plasmids required.
  • Pat:
    • Will transform, growup and prepare glycerol stocks of death plasmid in DB3.1 this week.
  • Kasia: Documentation progress vs last report/ new problems/ needs.-No report

6.15 New Agenda Items:

  • Phil: Individuals desired time commitment:
IndividualDesired time committment
Pat About 4h/wk Monday afternoon or Tuesday morning
Alisa About 4h/wk - Thursday afternoon
Jan About 10h/pw - flexible weekday mornings
Phil About 18h/pw - Thursday morning, Friday, Saturday or Sunday,
  • Phil: Administrative Timetable: (12 Weeks to go)
    • Fab request & project description by Tuesday 31 July.
    • "Produce and characterize buoyant bacteria and light receptor components of coliforming system."
  • Next meeting: <b/> 6th August (Monday) 1.15PM agreed but latter changed to 3rd Aug (Friday) 5:30PM.

6:30 End

Igem Administrative Timetable:

1 August Team project descriptions due.
1 September Final team rosters due.
1 September Jamboree attendance fees due $100USD per team

member.

26 October Project and part documentation due DNA received USA.
26 October BioBrick Part DNA received by the Registry.
3-4 November iGEM Competition Jamboree, MIT, USA.

.


Documentation wiki status:

    • Priority should be on plans and experimental report analysis and conclusions-----Alisa .
    • If only Pat & Alisa doing lab work not much documentation needed.
    • Equipement:
      • MissingLas3000 method (in lab notebook)
      • Missing DNA measurement protocol (eppendorf)
    • Reagents pages incomplete:
      • Some location pictures not finished or uploaded.
      • Some newer reagents not entered.
    • Secondary reagents pages not made:
      • LB, Plates, TAE , NZY+ Broth
    • Protocol part:
      • No connections from Protocols to reagents.
      • Ligation protocol missing
    • Lab notebook:
      • Format uses too many lines to see connections between days.
      • Latter part missing links
      • Can table of contents be restricted to date level?
    • Experimental reports:
      • Lacks discussion/conclusion
      • Ligation experiment missing
      • Lattest few gells not marked up and entered.
    • Project overview link broken
    • Background:
      • GFP missing ,
      • Red photo sensor to GFP test harness missing.
    • Plan: Only Gas vesicles plan entered.
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