Glasgow/Wetlab/Week6
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Maia checked the presence and size of (*m*) and (*s*) inserts within the Topo vector by repeating the PCR performed on 02/08. Results confirmed presence of inserts with the correct size. The TOPO vectors containing (*m*) and (*s*) were then sent for sequencing to ensure the inserts did not contain any mistakes. DNA sequenced and the primers used are summarized in the table below. | Maia checked the presence and size of (*m*) and (*s*) inserts within the Topo vector by repeating the PCR performed on 02/08. Results confirmed presence of inserts with the correct size. The TOPO vectors containing (*m*) and (*s*) were then sent for sequencing to ensure the inserts did not contain any mistakes. DNA sequenced and the primers used are summarized in the table below. | ||
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Revision as of 10:24, 16 August 2007
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Contents |
Week 6
Monday 6th August 2007
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Maia checked the presence and size of (*m*) and (*s*) inserts within the Topo vector by repeating the PCR performed on 02/08. Results confirmed presence of inserts with the correct size. The TOPO vectors containing (*m*) and (*s*) were then sent for sequencing to ensure the inserts did not contain any mistakes. DNA sequenced and the primers used are summarized in the table below.
Label B1 B3 C5 Gene (*m*) (*s*) (*m*) Colony 17,18,19,26 20 25,24 Primers (*m*)_for_1 + Methyl _2 (*s*)_for_1 + Oxy_2 (*m*)_for_1 + (*m*)_rev_1 Tuesday 7th August 2007
Wednesday 8th August 2007
Thursday 9th August 2007
=== Friday 10th August 2007 ===
