From 2007.igem.org
Click on a week number for more detailed lab book...
Week 1
| Week 2
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- First selection of biobricks transformed.
- Preparation of stocks.
|
- Researched DmpR, XylR and DntR sensor systems.
- Designed primers for DmpR, DntR, XylR and associated promoters.
|
Week 3
| Week 4
|
- BioBricks tested by digestion and PCR.
- PCR with new primers associated with DntR and XylR.
|
- phzM and phzS cloned into TOPO vectors.
- Primer design for 7 gene operon.
- DntR cloned into TOPO vector.
- Primer design for XylR and associated genes.
- Death gene cloned into TOPO vector.
- Primer design for death gene.
|
Week 5
| Week 6
|
- Suspected amplification of 7 gene operon.
- phzM and phzS confirmed as present in TOPO vectors.
- Confirmed presence of death gene plasmid in some vectors.
- Miller assay performed with DntR system.
- Yeast cells.
|
- Biobricks made of phzM, phzS, and DntR.
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Week 7
| Week 8
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- Utilised new biobricks.
- Site directed mutagenesis of phzM completed.
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- Site directed mutagenesis of phzS completed.
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Week 9
| Week 10+
|
- phzD→phzG successfully cloned into TOPO vector.
|
- Contains further work achieved.
- XylR and Pr cloned into construction vectors.
- XylR and Pr put next to responsive promoter and luciferase.
|
