Glasgow/Wetlab/Orders
From 2007.igem.org
(Difference between revisions)
(→Designed primers) |
(→Designed primers) |
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| Line 267: | Line 267: | ||
|61.3 | |61.3 | ||
|- | |- | ||
| - | | | + | |Bbp_XylR_for_2 |
|5´ GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC 3´ | |5´ GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC 3´ | ||
|55.2 | |55.2 | ||
|- | |- | ||
| - | | | + | |Bbs_XylR_rev_2 |
|5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG 3´ | |5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG 3´ | ||
|61.8 | |61.8 | ||
|- | |- | ||
| - | | | + | |Bbp_Pr_for_2 |
|5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG 3´ | |5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG 3´ | ||
|60.8 | |60.8 | ||
|- | |- | ||
| - | | | + | |Bbs_Pr_rev_2 |
|5´ CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC 3´ | |5´ CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC 3´ | ||
|61.3 | |61.3 | ||
|- | |- | ||
| - | | | + | |Bbp_Pu_for_2 |
|5´ GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC 3´ | |5´ GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC 3´ | ||
|64.3 | |64.3 | ||
|- | |- | ||
| - | | | + | |Bbs_Pu_rev_2 |
|5´ CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG 3´ | |5´ CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG 3´ | ||
|69.9 | |69.9 | ||
|- | |- | ||
| - | | | + | |Bbp_***D_for |
|5´ *** 3´ | |5´ *** 3´ | ||
|61.0 | |61.0 | ||
|- | |- | ||
| - | | | + | |Bbs_***D_for |
|5´ *** 3´ | |5´ *** 3´ | ||
|61.4 | |61.4 | ||
|- | |- | ||
| - | | | + | |Bbp_***A_for |
|5´ *** 3´ | |5´ *** 3´ | ||
|63.1 | |63.1 | ||
|- | |- | ||
| - | | | + | |Bbs_***G_rev |
|5´ *** 3´ | |5´ *** 3´ | ||
|61.9 | |61.9 | ||
|} | |} | ||
Revision as of 10:38, 16 August 2007
Emma's primers
| Name | Sequence | Tm |
|---|---|---|
| * ***Up | 5´ *** 3´ | 65.6 |
| * ***Low | 5´ *** 3´ | 64.8 |
| *Bbp_***Up | 5´ *** 3´ | 63.6 |
| *Bbs_***Low | 5´ *** 3´ | 64.2 |
| Bbp_Gene | 5´ gaattcgcggccgcttctag 3´ | 65.7 |
| Bbs | 5´ ctgcagcggccgctactagta 3´ | 65.3 |
| *Methyl_1 | 5´ *** 3´ | 66.3 |
| *Methyl_2 | 5´ *** 3´ | 66.2 |
| *Bbp_Methyl_1 | 5´ *** 3´ | 63.9 |
| *Bbs_Methyl_2 | 5´ *** 3´ | 66.9 |
| ***M_for_1 | 5´ *** 3´ | 65.4 |
| ***M_rev_1 | 5´ *** 3´ | 60.3 |
| Bbp_***M_for_1 | 5´ *** 3´ | 64.5 |
| Bbs_***M_rev_1 | 5´ *** 3´ | 60.3 |
| *Oxy_1 | 5´ *** 3´ | 60.2 |
| *Oxy_2 | 5´ *** 3´ | 59.2 |
| *Bbp_Oxy_1 | 5´ *** 3´ | 65.1 |
| *Bbs_Oxy_2 | 5´ *** 3´ | 66.9 |
| ***S_for_1 | 5´ *** 3´ | 71.2 |
| ***S_rev_1 | 5´ *** 3´ | 60.2 |
| Bbp_***S_for_1 | 5´ *** 3´ | 70.9 |
| Bbs_***S_rev_1 | 5´ *** 3´ | 63.3 |
| ***H_up | 5´ *** 3´ | 69.9 |
| ***H_low | 5´ *** 3´ | 70.6 |
| Bbp_***Up | 5´ *** 3´ | 63.3 |
| Bbs_***Low | 5´ *** 3´ | 57.7 |
Designed primers
| Name | Sequence | Tm |
|---|---|---|
| DmpR_SDM_PstI_1_for | 5´ CTGGAGCAACAGCATGCGCTGTTC 3´ | 65.6 |
| DmpR_SDM_PstI_1_rev | 5´ GCTCCAGTTTTCAGCGATGGCC 3´ | 64.8 |
| DmpR_SDM_PstI_2_for | 5´ CTGGAGGAAGTGCTCAACCAGCAG 3´ | 66.3 |
| DmpR_SDM_PstI_2_rev | 5´ CCTCCAGCGCTTCCACCAG 3´ | 66.2 |
| DmpR_Start_Prefix_1_for | 5´ GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG 3´ | 60.2 |
| DmpR_End_Suffix_1_rev | 5´ CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC 3´ | 59.2 |
| ***M_SDM_PstI_1_for | 5´ *** 3´ | 69.9 |
| ***M_SDM_PstI_1_rev | 5´ *** 3´ | 70.6 |
| ***S_SDM_PstI_1_for | 5´ *** 3´ | 65.7 |
| ***S_SDM_PstI_1_rev | 5´ *** 3´ | 65.3 |
| ***S_SDM_PstI_2_for | 5´ *** 3´ | 63.6 |
| ***S_SDM_PstI_2_rev | 5´ *** 3´ | 63.9 |
| ***C_SDM_PstI_1_for | 5´ *** 3´ | 65.1 |
| ***C_SDM_PstI_1_rev | 5´ *** 3´ | 64.2 |
| ***F_SDM_PstI_1_for | 5´ *** 3´ | 66.9 |
| ***F_SDM_PstI_1_rev | 5´ *** 3´ | 66.9 |
| ***B_SDM_EcoRI_1_for | 5´ *** 3´ | 65.4 |
| ***B_SDM_EcoRI_1_rev | 5´ *** 3´ | 64.5 |
| ***E_SDM_EcoRI_1_for | 5´ *** 3´ | 60.3 |
| ***E_SDM_EcoRI_1_rev | 5´ *** 3´ | 60.3 |
| ***E_SDM_PstI_1_for | 5´ *** 3´ | 71.2 |
| ***E_SDM_PstI_1_rev | 5´ *** 3´ | 70.9 |
| XylR_SDM_PstI_1_for | 5´ GAGCTCCAGACCCAGGTTG 3´ | 63.3 |
| XylR_SDM_PstI_1_rev | 5´ GTCTGGAGCTCGTAGCGCTC 3´ | 63.3 |
| XylR_Prefix_1 | 5´ GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG 3´ | 57.7 |
| XylR_Suffix_1 | 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC 3´ | 59.8 |
| Pu_Prefix_1(After XylR) | 5´ GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG 3´ | 65.0 |
| Pu_Suffix_1(After XylR) | 5´ CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG 3´ | 65.1 |
| Pu_Prefix_2(Emma´s sequence) | 5´ GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC 3´ | 66.0 |
| Pu_Suffix_2(Emma´s sequence) | 5´ CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC 3´ | 66.4 |
| DntR_Prefix_1 | 5´ GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG 3´ | 61.2 |
| DntR_Suffix_1 | 5´ TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT 3´ | 55.2 |
| DntR_Suffix_2 | 5´ CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG 3´ | 55.3 |
| Pr_Prefix_1 | 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG 3´ | 58.8 |
| Pr_Suffix_1 | 5´ CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG 3´ | 57.3 |
| p1010_for | 5´ GCTGTGTATAAGGGAGCCTGA 3´ | 59.4 |
| p1010_rev | 5´ TGACAGTTGACAGCGACAGC 3´ | 61.3 |
| Bbp_XylR_for_2 | 5´ GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC 3´ | 55.2 |
| Bbs_XylR_rev_2 | 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG 3´ | 61.8 |
| Bbp_Pr_for_2 | 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG 3´ | 60.8 |
| Bbs_Pr_rev_2 | 5´ CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC 3´ | 61.3 |
| Bbp_Pu_for_2 | 5´ GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC 3´ | 64.3 |
| Bbs_Pu_rev_2 | 5´ CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG 3´ | 69.9 |
| Bbp_***D_for | 5´ *** 3´ | 61.0 |
| Bbs_***D_for | 5´ *** 3´ | 61.4 |
| Bbp_***A_for | 5´ *** 3´ | 63.1 |
| Bbs_***G_rev | 5´ *** 3´ | 61.9 |
