Glasgow/Wetlab

From 2007.igem.org

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''Click on a week number for more detailed lab book...''
''Click on a week number for more detailed lab book...''
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{|cellspacing="6px" cellpadding="16" border="0" width="100%"
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|- align="center"
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!width="50%" | [https://2007.igem.org/Glasgow/Wetlab/Week1 https://static.igem.org/mediawiki/2007/c/ce/Week_1.png] <br>
 +
*First selection of biobricks transformed.
 +
*Preparation of stocks.
 +
 
 +
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week2 https://static.igem.org/mediawiki/2007/2/20/Week_2.png] <br>
 +
*Change of plan because DmpR failed to grow.
 +
*Researched XylR and DntR sensor systems.
 +
*Designed primers for DmpR, DntR, XylR and associated promoters.
 +
 
 +
|- align="center"
 +
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week3 https://static.igem.org/mediawiki/2007/7/74/Week_3.png] <br>
 +
*BioBricks tested by digestion and PCR.
 +
*PCR with new primers associated with DntR and XylR.
 +
 
 +
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week4 https://static.igem.org/mediawiki/2007/3/34/Week_4.png] <br>
 +
**m and *s cloned into TOPO vectors.
 +
*Primer design for 7 gene operon.
 +
*DntR cloned into TOPO vector.
 +
*Primer design for XylR and associated genes.
 +
*Death gene cloned into TOPO vector.
 +
*Primer design for death gene.
 +
 
|- align="left"
|- align="left"
-
!width="180" | [https://2007.igem.org/Glasgow/Wetlab/Week1 https://static.igem.org/mediawiki/2007/c/ce/Week_1.png] || First selection of biobricks transformed. <br>Preparation of stocks.
+
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week5 https://static.igem.org/mediawiki/2007/4/4f/Week_5.png] <br>
 +
*Suspected amplification of 7 gene operon.
 +
**m and *s confirmed as present in TOPO vectors.
 +
*Confirmed presence of death gene plasmid in some vectors.
 +
*Miller assay performed with DntR system.
 +
*Yeast cells.
 +
 
 +
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week6 https://static.igem.org/mediawiki/2007/7/70/Week_6.png] <br>
 +
*'''''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''''
|- align="left"
|- align="left"
-
!width="180" |[https://2007.igem.org/Glasgow/Wetlab/Week2 https://static.igem.org/mediawiki/2007/2/20/Week_2.png] ||
+
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week7 https://static.igem.org/mediawiki/2007/c/c0/Week_7.png] <br>  
-
<br>Change of plan because DmpR failed to grow.
+
*Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
-
<br>Researched XylR and DntR sensor systems.
+
 
-
<br>Designed primers for DmpR, DntR, XylR and associated promoters.
+
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week8 https://static.igem.org/mediawiki/2007/9/9b/Week_8.png] <br>  
-
|- align="left"
+
*'''''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''''
-
!width="180" |[https://2007.igem.org/Glasgow/Wetlab/Week3 https://static.igem.org/mediawiki/2007/7/74/Week_3.png] ||
+
 
-
<br>BioBricks tested by digestion and PCR.
+
-
<br>PCR with new primers associated with DntR and XylR.
+
|- align="left"
|- align="left"
-
!width="180" |[https://2007.igem.org/Glasgow/Wetlab/Week4 https://static.igem.org/mediawiki/2007/3/34/Week_4.png] ||
+
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week9 https://static.igem.org/mediawiki/2007/a/a1/Week_9.png] <br>  
-
<br>*m and *s cloned into TOPO vectors.
+
*Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
-
<br>Primer design for 7 gene operon.
+
 
-
<br>DntR cloned into TOPO vector.
+
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week10 https://static.igem.org/mediawiki/2007/8/88/Week_10.png] <br>
-
<br>Primer design for XylR and associated genes.
+
*'''''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''''
-
<br>Death gene cloned into TOPO vector.
+
-
<br>Primer design for death gene.
+
-
|- align="left"
+
-
!width="180" |[https://2007.igem.org/Glasgow/Wetlab/Week5 https://static.igem.org/mediawiki/2007/4/4f/Week_5.png] ||
+
-
<br>Suspected amplification of 7 gene operon.
+
-
<br>*m and *s confirmed as present in TOPO vectors.
+
-
<br>Confirmed presence of death gene plasmid in some vectors.
+
-
<br>Miller assay performed with DntR system.
+
-
<br>Yeast cells.
+
-
|- align="left"
+
-
!width="180" |[https://2007.igem.org/Glasgow/Wetlab/Week6 https://static.igem.org/mediawiki/2007/7/70/Week_6.png] || '''''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''''
+
-
|- align="left"
+
-
!width="180" |[https://2007.igem.org/Glasgow/Wetlab/Week7 https://static.igem.org/mediawiki/2007/c/c0/Week_7.png] || Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
-
|- align="left"
+
-
!width="180" |[https://2007.igem.org/Glasgow/Wetlab/Week8 https://static.igem.org/mediawiki/2007/9/9b/Week_8.png] || '''''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''''
+
-
|- align="left"
+
-
!width="180" |[https://2007.igem.org/Glasgow/Wetlab/Week9 https://static.igem.org/mediawiki/2007/a/a1/Week_9.png] || Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
-
|- align="left"
+
-
!width="180" |[https://2007.igem.org/Glasgow/Wetlab/Week10 https://static.igem.org/mediawiki/2007/8/88/Week_10.png] || '''''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''''
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|}
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Revision as of 19:25, 24 August 2007

Glasgow Main Page

PROTOCOLS REFERENCES RESOURCES ORDERS BIOBRICKS UTILISED GELS


Click on a week number for more detailed lab book...


https://static.igem.org/mediawiki/2007/c/ce/Week_1.png
  • First selection of biobricks transformed.
  • Preparation of stocks.
https://static.igem.org/mediawiki/2007/2/20/Week_2.png
  • Change of plan because DmpR failed to grow.
  • Researched XylR and DntR sensor systems.
  • Designed primers for DmpR, DntR, XylR and associated promoters.
https://static.igem.org/mediawiki/2007/7/74/Week_3.png
  • BioBricks tested by digestion and PCR.
  • PCR with new primers associated with DntR and XylR.
https://static.igem.org/mediawiki/2007/3/34/Week_4.png
    • m and *s cloned into TOPO vectors.
  • Primer design for 7 gene operon.
  • DntR cloned into TOPO vector.
  • Primer design for XylR and associated genes.
  • Death gene cloned into TOPO vector.
  • Primer design for death gene.
https://static.igem.org/mediawiki/2007/4/4f/Week_5.png
  • Suspected amplification of 7 gene operon.
    • m and *s confirmed as present in TOPO vectors.
  • Confirmed presence of death gene plasmid in some vectors.
  • Miller assay performed with DntR system.
  • Yeast cells.
https://static.igem.org/mediawiki/2007/7/70/Week_6.png
  • Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
https://static.igem.org/mediawiki/2007/c/c0/Week_7.png
  • Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
https://static.igem.org/mediawiki/2007/9/9b/Week_8.png
  • Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
https://static.igem.org/mediawiki/2007/a/a1/Week_9.png
  • Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
https://static.igem.org/mediawiki/2007/8/88/Week_10.png
  • Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
PROTOCOLS REFERENCES ORDERS BIOBRICKS UTILISED GELS
Retrieved from "http://2007.igem.org/wiki/index.php/Glasgow/Wetlab"