From 2007.igem.org

Revision as of 13:48, 21 August 2007 (view source) Landrain (Talk | contribs) (→Growth kinetic on w121 strains) ← Older edit		Latest revision as of 13:27, 27 August 2007 (view source) Landrain (Talk | contribs) (→Culture of w121 and FX85 strains for preparing chemiocompetent cells on tomorrow)
(6 intermediate revisions not shown)
Line 1:		Line 1:
-	== Growth kinetic on w121 strains ==	+	[[Paris/August 20|yesterday]] -- [[Paris/August 22|tomorrow]]
-	{{Paris_KineticArray| Title = w121 kinetic as a function of DAP and supplemented medium (S0.x)	+	== Culture of w121 and FX85 strains for preparing chemiocompetent cells on tomorrow ==
-	|A1=H<sub>2</sub>0|A2=H<sub>2</sub>0|A3=H<sub>2</sub>0|A4=H<sub>2</sub>0	+
-	|A5=H<sub>2</sub>0|A6=H<sub>2</sub>0|A7=H<sub>2</sub>0|A8=H<sub>2</sub>0	+	We spread LB with W121 (Erythromycine; DAP) and FX85 (Kanamycine; Thymine)
-	|A9=H<sub>2</sub>0|A10=H<sub>2</sub>0|A11=H<sub>2</sub>0|A12=H<sub>2</sub>0	+
-	|B1=H<sub>2</sub>0	+	== Culture of Ligation Strains ==
-	|B2=LB+0µM DAP|B3=LB+5µM DAP|B4=LB+8µM DAP|B5=LB+10µM DAP|B6=LB+12µM DAP	+
-	|B7=LB+15µM DAP|B8=LB+17µM DAP|B9=LB+20µM DAP|B10=LB+30µM DAP|B11=LB+40µM DAP	+	We made 5mL culture of the new ligation strains for future minipreps and glycerol stocks.<br>
-	|B12=H<sub>2</sub>0|C1=H<sub>2</sub>0	+	Here are the Ligation strains put in culture.<br>
-	|C2=S0.2+0µM DAP|C3=S0.2+5µM DAP|C4=S0.2+6µM DAP|C5=S0.2+7µM DAP|C6=S0.2+8µM DAP	+
-	|C7=S0.2+9µM DAP|C8=S0.2+10µM DAP|C9=S0.2+15µM DAP|C10=S0.2+17µM DAP|C11=S0.2+20µM DAP	+	*L31.1 - .3 - .4
-	|C12=H<sub>2</sub>0|D1=H<sub>2</sub>0	+	*L32.1 - .2 - .3
-	|D2=S0.4+0µM DAP|D3=S0.4+2µM DAP|D4=S0.4+3µM DAP|D5=S0.4+4µM DAP|D6=S0.4+5µM DAP	+	*L34.1 - .2 - .3
-	|D7=S0.4+8µM DAP|D8=S0.4+10µM DAP|D9=S0.4+12µM DAP|D10=S0.4+15µM DAP|D11=S0.4+20µM DAP	+	*L35.1 - .2 - .3
-	|D12=H<sub>2</sub>0|E1=H<sub>2</sub>0	+	*L36.1 - .2 - .3
-	|E2=S0.6+0µM DAP|E3=S0.6+1µM DAP|E4=S0.6+2µM DAP|E5=S0.6+3µM DAP|E6=S0.6+4µM DAP	+	*L37.1 - .2 - .3
-	|E7=S0.6+5µM DAP|E8=S0.6+6µM DAP|E9=S0.6+7µM DAP|E10=S0.6+8µM DAP|E11=S0.6+10µM DAP	+	*L39.1 - .2 - .3
-	|E12=H<sub>2</sub>0|F1=H<sub>2</sub>0	+	*L40.1 - .2
-	|F2=S0.8+0µM DAP|F3=S0.8+0,5µM DAP|F4=S0.8+1µM DAP|F5=S0.8+2µM DAP|F6=S0.8+3µM DAP	+	*L41.1
-	|F7=S0.8+4µM DAP|F8=S0.8+5µM DAP|F9=S0.8+6µM DAP|F10=S0.8+7µM DAP|F11=S0.8+10µM DAP	+	*L42.1 - .2 - .3
-	|F12=H<sub>2</sub>0|G1=H<sub>2</sub>0	+	*L43.1 - .2 - .3
-	|G2=H<sub>2</sub>0|G3=H<sub>2</sub>0|G4=H<sub>2</sub>0|G5=H<sub>2</sub>0|G6=H<sub>2</sub>0	+
-	|G7=H<sub>2</sub>0|G8=H<sub>2</sub>0|G9=H<sub>2</sub>0|G10=H<sub>2</sub>0|G11=H<sub>2</sub>0	+
-	|G12=H<sub>2</sub>0|H1=H<sub>2</sub>0|H2=H<sub>2</sub>0|H3=H<sub>2</sub>0|H4=H<sub>2</sub>0	+
-	|H5=H<sub>2</sub>0|H6=H<sub>2</sub>0|H7=H<sub>2</sub>0|H8=H<sub>2</sub>0	+
-	|H9=H<sub>2</sub>0|H10=H<sub>2</sub>0|H11=H<sub>2</sub>0|H12=H<sub>2</sub>0}}	+

---

Latest revision as of 13:27, 27 August 2007

yesterday -- tomorrow

Culture of w121 and FX85 strains for preparing chemiocompetent cells on tomorrow

We spread LB with W121 (Erythromycine; DAP) and FX85 (Kanamycine; Thymine)

Culture of Ligation Strains

We made 5mL culture of the new ligation strains for future minipreps and glycerol stocks.
Here are the Ligation strains put in culture.

• L31.1 - .3 - .4
• L32.1 - .2 - .3
• L34.1 - .2 - .3
• L35.1 - .2 - .3
• L36.1 - .2 - .3
• L37.1 - .2 - .3
• L39.1 - .2 - .3
• L40.1 - .2
• L41.1
• L42.1 - .2 - .3
• L43.1 - .2 - .3