From 2007.igem.org

Revision as of 13:36, 27 August 2007 (view source) Francesca.bugane2 (Talk | contribs) ← Older edit		Revision as of 13:36, 27 August 2007 (view source) Francesca.bugane2 (Talk | contribs) Newer edit →
Line 29:		Line 29:
	::'''08/24/07'''		::'''08/24/07'''
-		+	*We plane new amplifications.
	[[Bologna | Back]]		[[Bologna | Back]]

---

Revision as of 13:36, 27 August 2007

08/20/07

• I763010 and C0012 trasformation.

08/21/07

• There isn't colonies for I763010 and C0012. we can't amplify this biobrick.
• J04631 digestion.
• Band extraction.
• R0051+P0412 (I763010)ligation.

08/22/07

• There isn't any colony for R0051+P0412 (I763010)

08/23/07

• A new R0051+P0412 (I763010) ligation a new protocol (3h 15°C and 1.2 ul Ligasi).
• R0051, I763012 digestion with Spe/Pst1.
• P0412, J04631 digestion with Xba/Pst1.
• Run and extraction from gel.
• Trasformation of R0051+P0412 (I763010) ligations.

08/24/07

• We plane new amplifications.

Back