NYMU Taipei/Lab Notes/2007 8 29
From 2007.igem.org
(Difference between revisions)
Lihsiangyen (Talk | contribs) |
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**Emition: 527nm | **Emition: 527nm | ||
*Liquid culture for MGC clones | *Liquid culture for MGC clones | ||
| - | ** INS(human), IGFBP3(human), IGFBP7(human), IDE(mouse) for insulin project | + | ** INS (human), IGFBP3 (human), IGFBP7 (human), IDE (mouse) for insulin project |
| - | ** PUM1, AKAP1 for m.t. project | + | ** PUM1 (human), AKAP1 (human) for m.t. project |
---- | ---- | ||
by 立翔 | by 立翔 | ||
Revision as of 07:05, 30 August 2007
- Plasmid extraction:
- pOmpC+EYFP 1-1, 1-2, 2, 3, 4, 5 (duplicate the first tube for even balance)
- Extract from 1.5ml LB medium to 20ul EB buffer
- Stored in -20°C
- EYFP spectral characteristics:
- Excitation: 514nm
- Emition: 527nm
- Liquid culture for MGC clones
- INS (human), IGFBP3 (human), IGFBP7 (human), IDE (mouse) for insulin project
- PUM1 (human), AKAP1 (human) for m.t. project
by 立翔
