Week 10

From 2007.igem.org

(Difference between revisions)
Line 24: Line 24:
*Ligation for:
*Ligation for:
-
-[http://partsregistry.org/Part:BBa_I763025 I763025]+http://partsregistry.org/Part:BBa_B0015(http://partsregistry.org/Part:BBa_I763026);
+
-[http://partsregistry.org/Part:BBa_I763025 I763025]+[http://partsregistry.org/Part:BBa_B0015 B0015]([http://partsregistry.org/Part:BBa_I763026 I763026]);
-
-http://partsregistry.org/Part:BBa_I763024+http://partsregistry.org/Part:BBa_B0015(http://partsregistry.org/Part:BBa_I763030);
+
-[http://partsregistry.org/Part:BBa_I763024 I763024] +[http://partsregistry.org/Part:BBa_B0015 B0015]([http://partsregistry.org/Part:BBa_I763030 I763030]);
-
-http://partsregistry.org/Part:BBa_I763016+http://partsregistry.org/Part:BBa_I763020(http://partsregistry.org/Part:BBa_I763019);
+
-[http://partsregistry.org/Part:BBa_I763016 I763016]+[http://partsregistry.org/Part:BBa_I763020 I763020]([http://partsregistry.org/Part:BBa_I763019 I763019]);
-
-http://partsregistry.org/Part:BBa_I763009+http://partsregistry.org/Part:BBa_P0412(http://partsregistry.org/Part:BBa_I763021);
+
-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_P0412 P0412]([http://partsregistry.org/Part:BBa_I763021 I763021]);
-
-I763009+S0100(I763023);
+
-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_S0100 S0100]([http://partsregistry.org/Part:BBa_I763023 I763023]);
-
-I763009+S03520(I763022);
+
-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_S03520 S03520]([http://partsregistry.org/Part:BBa_I763022 I763022]);
-
-R0051+I763020(I763011);
+
-[http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_I763020 I763020]([http://partsregistry.org/Part:BBa_I763011 I763011]);

Revision as of 20:31, 7 September 2007

09/03/07
  • Miniprep for:

-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S0100 S0100];

-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S03520 S03520];

-[http://partsregistry.org/Part:BBa_I763005 I763005]+[http://partsregistry.org/Part:BBa_I763015 I763015];

-[http://partsregistry.org/Part:BBa_J52034 J52034]+[http://partsregistry.org/Part:BBa_E0022 E0022];

  • Digestion for:

-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S0100 S0100] with Eco/Spe;

-[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_S03520 S03520] with Eco/Spe;

-[http://partsregistry.org/Part:BBa_I763005 I763005]+[http://partsregistry.org/Part:BBa_I763015 I763015] with Spe/Pst1;

-[http://partsregistry.org/Part:BBa_J52034 J52034]+[http://partsregistry.org/Part:BBa_E0022 E0022] with Spe/Pst1;

-[http://partsregistry.org/Part:BBa_B0015 B0015] with Eco/Xba;

  • Band extraction;
  • Ligation for:

-[http://partsregistry.org/Part:BBa_I763025 I763025]+[http://partsregistry.org/Part:BBa_B0015 B0015]([http://partsregistry.org/Part:BBa_I763026 I763026]);

-[http://partsregistry.org/Part:BBa_I763024 I763024] +[http://partsregistry.org/Part:BBa_B0015 B0015]([http://partsregistry.org/Part:BBa_I763030 I763030]);

-[http://partsregistry.org/Part:BBa_I763016 I763016]+[http://partsregistry.org/Part:BBa_I763020 I763020]([http://partsregistry.org/Part:BBa_I763019 I763019]);

-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_P0412 P0412]([http://partsregistry.org/Part:BBa_I763021 I763021]);

-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_S0100 S0100]([http://partsregistry.org/Part:BBa_I763023 I763023]);

-[http://partsregistry.org/Part:BBa_I763009 I763009]+[http://partsregistry.org/Part:BBa_S03520 S03520]([http://partsregistry.org/Part:BBa_I763022 I763022]);

-[http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_I763020 I763020]([http://partsregistry.org/Part:BBa_I763011 I763011]);


09/04/07
  • There are colonies for every ligations.
  • Meeting.


09/05/07

Miniprep for:

-I763016+I763020(I763019);

-I763025+B0015(I763026);

-I763024+B0015(I763030);

-R0051+I763020(I763011);

-I763009+P0412(I763021);

-I763009+S03520(I763022);

  • Digestion for:

-I763019 with Eco/Xba;

-I763026 with Eco/Spe;

-I763030 with Eco/Spe;

-I763011 with Xba/Pst1;

-R0051+I13507(I763007) with Xba/Pst1;

-I763021 with Eco/Spe;

  • Control digestion for I763019 with Eco/Pst1;
  • Band extraction for

-I763019;

-I763026;

-I763011;

-R0051+I13507(I763007);

  • New control digestion for:

-I763019 with Xba/Spe;

-I763019 with Xba/Pst1;

-I763021 with Eco/Spe;

-I763026 with Eco/Spe;

-I763030 with Eco/Spe;

  • Band extraction for I763019 (digested with Xba/Spe and with Xba/Pst1), I763026;
  • Ligation O/N for:

-I763026+I763019(I763028);

-I763026+I763004(I763027);


09/06/07

Digestion for:

-I763019 with Eco/Xba;

-I763021 with Eco/Spe;

-I763026 with Eco/Spe.

  • Band extraction from electrophoresis gel for I763019 and I763026.
  • Ligation for I763027 with new extracts.
  • Transformations for the new ligations.
09/07/07

Back

Retrieved from "http://2007.igem.org/wiki/index.php/Week_10"