Restriction Enzyme
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| - | We have selected HindIII and BamHI for promoter insertion and BamHI and SacI for firefly luciferase insertion. | + | We have selected HindIII and BamHI for promoter insertion and BamHI and SacI for firefly luciferase and coding sequences insertion. |
| + | Restriction enzymes are named based on the bacteria in which they are isolated in the following manner | ||
| + | {| border="1" | ||
| + | !Enzyme!!Source!!Recognition Sequence!!Cut | ||
| + | |- | ||
| + | |[[BamHI|''Bam''HI]]||''[[Bacillus amyloliquefaciens]]'' | ||
| + | | | ||
| + | 5'GGATCC | ||
| + | 3'CCTAGG | ||
| + | | | ||
| + | 5'---G GATCC---3' | ||
| + | 3'---CCTAG G---5' | ||
| + | |- | ||
| + | |[[HindIII|''Hin''dIII]]||''[[Haemophilus influenzae]]'' | ||
| + | | | ||
| + | 5'AAGCTT | ||
| + | 3'TTCGAA | ||
| + | | | ||
| + | 5'---A AGCTT---3' | ||
| + | 3'---TTCGA A---5' | ||
| + | |- | ||
| + | |[[SacI|''Sac''I]]||''[[Streptomyces achromogenes]]'' | ||
| + | | | ||
| + | 5'GAGCTC | ||
| + | 3'CTCGAG | ||
| + | | | ||
| + | 5'---GAGCT C---3' | ||
| + | 3'---C TCGAG---5' | ||
| + | |- | ||
| + | |} | ||
Latest revision as of 15:42, 18 September 2007
We have selected HindIII and BamHI for promoter insertion and BamHI and SacI for firefly luciferase and coding sequences insertion. Restriction enzymes are named based on the bacteria in which they are isolated in the following manner
| Enzyme | Source | Recognition Sequence | Cut |
|---|---|---|---|
| BamHI | Bacillus amyloliquefaciens |
5'GGATCC 3'CCTAGG |
5'---G GATCC---3' 3'---CCTAG G---5' |
| HindIII | Haemophilus influenzae |
5'AAGCTT 3'TTCGAA |
5'---A AGCTT---3' 3'---TTCGA A---5' |
| SacI | Streptomyces achromogenes |
5'GAGCTC 3'CTCGAG |
5'---GAGCT C---3' 3'---C TCGAG---5' |
