NYMU Taipei/Lab Notes/2007 9 19
From 2007.igem.org
(Difference between revisions)
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* GEL re-check for PCR annealing temperature gradient (load ? uL) | * GEL re-check for PCR annealing temperature gradient (load ? uL) | ||
<table border=1 align=center> | <table border=1 align=center> | ||
| - | <tr align=center><td>GEL check</td><td>ImageJ check at 122 bp | + | <tr align=center><td>GEL check</td><td>ImageJ check at 122 bp</td></tr> |
<tr align=center> | <tr align=center> | ||
<td>[[Image:NYMU Taipei 20070919 INS A PCR GEL check.jpg]]</td> | <td>[[Image:NYMU Taipei 20070919 INS A PCR GEL check.jpg]]</td> | ||
| Line 8: | Line 8: | ||
</table> | </table> | ||
* lanes from left to right | * lanes from left to right | ||
| - | ** ladder, 49.8 | + | ** ladder, 49.8 C, 52.3 C, 55.5 C, 58.7 C, 60.9 C, 55.5 C (control with template) |
---- | ---- | ||
* ligation for (CinR+HSL) + D-term | * ligation for (CinR+HSL) + D-term | ||
Revision as of 10:06, 19 September 2007
- GEL re-check for PCR annealing temperature gradient (load ? uL)
| GEL check | ImageJ check at 122 bp |
 |
 |
- lanes from left to right
- ladder, 49.8 C, 52.3 C, 55.5 C, 58.7 C, 60.9 C, 55.5 C (control with template)
- ligation for (CinR+HSL) + D-term
