Melbourne/meeting 14 September 2007

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== 5:30 Action points from last meeting:==
== 5:30 Action points from last meeting:==
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*Pat & Alisa: LacZ reporter plan - complete
 
*Jan: Plan for blue light on wiki by 17th August- Done but needs more work: couldn’t get anyone else to implement it based on this!
*Jan: Plan for blue light on wiki by 17th August- Done but needs more work: couldn’t get anyone else to implement it based on this!
-
 
-
*Phil: re prism :Edmund optics:VIS60  Schott veril linear variable interference filter SGD$1600 25mm wide 60mm long linear 400nm-700nm (contact sheet)
 
-
N47-279 eqilateral prism 35mm long 35mm face SGD$162
 
-
 
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*Phil: Chase Status of #BBa-M30109 & #BBa-V1012 - Arrived
 
-
 
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*Craig: Alkaline lysis technique for miniprep to be documented
 
-
 
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*Craig: Protein extraction technique –done sent to Phil by email.
 
-
 
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*Cheng: Daisy to produce competent cells of DB3.1 and Enz- strains: Alisa will set up plates for Daisy to work from by monday so we will have a store of competant cells by Tues/Wed
 
-
 
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*Chris: Gas vesicle expression experiment by 21/8/07.-Done
 
-
 
-
 
== 5:35 Status Reports: (5 minutes each including any discussion)==
== 5:35 Status Reports: (5 minutes each including any discussion)==
-
*Alisa:  Ligation experiments progress  
+
*Pat :  Red light receptor progress/design/plan- needs?
-
**Craig ligated the ompR promoter to the RBS-LacZ-Ter and c1 inverter, confirmation digest and gel to be done on saturday and parts sent for sequencing confirmation on Monday. 
+
**M30109: Must construct from scratch plan to be produced by Pat.
-
**Additional ligations to be set up and confirmed during the week: OmpR test harness(Ompr-RBS-LacZ-ter) to be ligated behind M30109, Ompr-c1 inverter to be ligated behind M30109.
+
**This is then drives RBS-ComP-term
 +
**Also required is ConstitutiveProm-RBS-ComA-term
-
*Pat & Alisa:  Red light receptor progress/design/plan- needs?
 
-
**these include to above ligations.  We need to work out how we are going to test these parts
 
-
*Jan: Blue light receptor design/plan progress - needs? (Absent from meeting)
+
*Jan & Alisa: Blue light receptor design/plan progress - needs?  
 +
**Primers ComA and Com P all in
 +
**Rapid Ligase 5min purchased
 +
**ComP and SopII to be conbined at variouse points by using 4 primer stiching:
 +
***Comp P forward
 +
***SopII reverse
 +
***ComP-SoPII overlap sense
 +
***ComP-SopII overlap antisense
 +
**Note that there are 7 different stiching pairs that result in combination at different points.
 +
**The product is then combined with RBS
 +
**Then transcription terminator
 +
**The constitutive promotor to create protein generator part.
 +
**Also Required is reporter construct PsfPromoter-RBS-LacZ-term
*Phil: Gas vesicles progress/design/plan - needs?
*Phil: Gas vesicles progress/design/plan - needs?
-
**Progress good, now on round 3 of mutagenesis (3 plates x2).
+
**Primers for part creation in
-
**Need miniprep of six colonies on Monday and Digest with PstI, I will pick colonies Sunday.
+
**No Progress , round 4 of mutagenesis plated awaits culture and diggest(4 plates).
-
**Buoyancy experiment confused re IPTG, -IPTG,+IPTG but definitely differences.  See attached photos at 1hour,6hours,18hours,96hours and last 3 at 126hours
+
**Buoyancy experiment needs repeating
**Other parts required from kit->glycerol stocks: B034 (P1-3O) , B0015 (P1-1I or P3-3O), J61034 (P4-8F or 8H)
**Other parts required from kit->glycerol stocks: B034 (P1-3O) , B0015 (P1-1I or P3-3O), J61034 (P4-8F or 8H)
-
 
+
**Pat to provide these.
==5.55 New Agenda Items:==
==5.55 New Agenda Items:==
-
*Phil: Administrative Timetable: (8 Weeks to go)
+
*Phil: Administrative Timetable: (5 Weeks to go)
-
*Final Team roster and Jamboree attendance fees due. Who is going?  Jan & Phil perhaps, others???  Need to find out state of funds so that we can work out how many people we are ''able'' to send
 
-
*Chris: Next years Comp Plan/preparations - same state as last week
 
-
*Any other business
+
*Chris: Next years Comp Plan/preparations - no progress
-
* Next meeting: <b/> 28th August (Friday) 5.30PM.
+
 
 +
* Next meeting: <b/> 21th September (Friday) 5.30PM.
==6:15 End ==
==6:15 End ==
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{|border="1"
{|border="1"
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|1 September ||              Final team rosters due.   
+
|1 October ||              Final team rosters due.   
|-
|-
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|1 September||              Jamboree attendance fees due  $100USD per team member.  
+
|1 October||              Jamboree attendance fees due  $100USD per team member.  
|-
|-
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|26 October||      Project and part documentation due DNA received USA.
+
|26 October||      Project and wiki & part documentation frozen, DNA received USA.
-
 
+
-
|-
+
-
 
+
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|26 October  ||    BioBrick Part DNA received by the Registry. 
+
|-
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Line 87: Line 76:
   
   
-
   
+
*re prism :Edmund optics:VIS60 Schott veril linear variable interference filter SGD$1600 25mm wide 60mm long linear 400nm-700nm (contact sheet)
 +
N47-279 eqilateral prism 35mm long 35mm face SGD$162
   
   

Latest revision as of 02:50, 20 September 2007

Contents

5:30 Action points from last meeting:

  • Jan: Plan for blue light on wiki by 17th August- Done but needs more work: couldn’t get anyone else to implement it based on this!

5:35 Status Reports: (5 minutes each including any discussion)

  • Pat : Red light receptor progress/design/plan- needs?
    • M30109: Must construct from scratch plan to be produced by Pat.
    • This is then drives RBS-ComP-term
    • Also required is ConstitutiveProm-RBS-ComA-term


  • Jan & Alisa: Blue light receptor design/plan progress - needs?
    • Primers ComA and Com P all in
    • Rapid Ligase 5min purchased
    • ComP and SopII to be conbined at variouse points by using 4 primer stiching:
      • Comp P forward
      • SopII reverse
      • ComP-SoPII overlap sense
      • ComP-SopII overlap antisense
    • Note that there are 7 different stiching pairs that result in combination at different points.
    • The product is then combined with RBS
    • Then transcription terminator
    • The constitutive promotor to create protein generator part.
    • Also Required is reporter construct PsfPromoter-RBS-LacZ-term
  • Phil: Gas vesicles progress/design/plan - needs?
    • Primers for part creation in
    • No Progress , round 4 of mutagenesis plated awaits culture and diggest(4 plates).
    • Buoyancy experiment needs repeating
    • Other parts required from kit->glycerol stocks: B034 (P1-3O) , B0015 (P1-1I or P3-3O), J61034 (P4-8F or 8H)
    • Pat to provide these.

5.55 New Agenda Items:

  • Phil: Administrative Timetable: (5 Weeks to go)


  • Chris: Next years Comp Plan/preparations - no progress


  • Next meeting: <b/> 21th September (Friday) 5.30PM.

6:15 End

Igem Administrative Timetable:

1 October Final team rosters due.
1 October Jamboree attendance fees due $100USD per team member.
26 October Project and wiki & part documentation frozen, DNA received USA.
3-4 November iGEM Competition Jamboree, MIT, USA.


.


  • re prism :Edmund optics:VIS60 Schott veril linear variable interference filter SGD$1600 25mm wide 60mm long linear 400nm-700nm (contact sheet)

N47-279 eqilateral prism 35mm long 35mm face SGD$162


Documentation wiki status:

    • Priority should be on plans and experimental report analysis and conclusions-----Alisa .
    • If only Pat & Alisa doing lab work not much documentation needed.
    • Equipement:
      • MissingLas3000 method (in lab notebook)
      • Missing DNA measurement protocol (eppendorf)
    • Reagents pages incomplete:
      • Some location pictures not finished or uploaded.
      • Some newer reagents not entered.
    • Secondary reagents pages not made:
      • LB, Plates, TAE , NZY+ Broth
    • Protocol part:
      • No connections from Protocols to reagents.
      • Ligation protocol missing
    • Lab notebook:
      • Format uses too many lines to see connections between days.
      • Latter part missing links
      • Can table of contents be restricted to date level?
    • Experimental reports:
      • Lacks discussion/conclusion
      • Ligation experiment missing
      • Lattest few gells not marked up and entered.
    • Project overview link broken
    • Background:
      • GFP missing ,
      • Red photo sensor to GFP test harness missing.
    • Plan: Only Gas vesicles plan entered.


IndividualDesired time committment
Pat About 4h/wk Monday afternoon or Tuesday morning
Alisa About 4h/wk - Thursday afternoon
Jan About 10h/pw - flexible weekday mornings
Phil About 18h/pw - Thursday morning, Friday, Saturday or Sunday,
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