NYMU Taipei/Lab Notes/2007 9 19
From 2007.igem.org
< NYMU Taipei/Lab Notes(Difference between revisions)
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| - | * GEL re-check for PCR gradient (load | + | * GEL re-check for INS_A PCR annealing temperature gradient (load 10 uL, 2 uL dye) |
<table border=1 align=center> | <table border=1 align=center> | ||
| - | <tr><td>GEL check</td><td>ImageJ check at 122 bp</td></tr> | + | <tr align=center><td>GEL check</td><td>ImageJ check at 122 bp</td></tr> |
| - | <tr> | + | <tr align=center> |
<td>[[Image:NYMU Taipei 20070919 INS A PCR GEL check.jpg]]</td> | <td>[[Image:NYMU Taipei 20070919 INS A PCR GEL check.jpg]]</td> | ||
<td>[[Image:NYMU Taipei 20070919 INS A PCR plot check.jpg]]</td> | <td>[[Image:NYMU Taipei 20070919 INS A PCR plot check.jpg]]</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
| + | * lanes from left to right | ||
| + | ** 100 bp ladder, 49.8 °C, 52.3 °C, 55.5 °C, 58.7 °C, 60.9 °C, 55.5 °C (control with template) | ||
| + | ---- | ||
* ligation for (CinR+HSL) + D-term | * ligation for (CinR+HSL) + D-term | ||
Latest revision as of 04:37, 20 September 2007
- GEL re-check for INS_A PCR annealing temperature gradient (load 10 uL, 2 uL dye)
| GEL check | ImageJ check at 122 bp |
 |
 |
- lanes from left to right
- 100 bp ladder, 49.8 °C, 52.3 °C, 55.5 °C, 58.7 °C, 60.9 °C, 55.5 °C (control with template)
- ligation for (CinR+HSL) + D-term
