Edinburgh/Yoghurt

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Revision as of 12:55, 23 September 2007

Edinburgh > Yoghurt

Introduction | Applications | Design | Status | References

Introduction | Applications | Design | Status | References

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 [https://2007.igem.org/Edinburgh https://static.igem.org/mediawiki/2007/f/f5/800px-Edinburgh_City_15_mod.JPG]
-=Self flavouring yoghurt=
-==Project Discription==
-This project looks at applying synthetic biology techniques to Gram positive organisms for special applications. In cases such as environmental applications, vaccines, and probiotics, it would be advantageous to use alternative bacterial hosts than the traditional E.Coli and budding yeast. As a proof of concept we are attemting to engineer lactic acid bacteria, such as ''Lactobacillus acidiphilus'' to synthesise flavours during yoghurt production.
-The idea is to stimulate the synthesis of a certain flavour and colour via the addition of an external stimulus.  Due to the nature of the finished product we will be using promoters induced by the disaccharides maltose, trehalose and arabinose.
-The project will be carried out in two parts.  The first is to generate the flavour and colour biosynthesis pathways in the well characterised host bacterium E. coli. The other part is to produce a way of placing biobricks into ''Lactobacillus''.
-Finally the flavour and colour biosynthesis pathways can be transferred to ''Lactobacillus''.
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