From 2007.igem.org

Revision as of 06:58, 12 July 2007 (view source) PhillipDodson (Talk | contribs) ← Older edit		Revision as of 08:20, 28 September 2007 (view source) Craig (Talk | contribs) Newer edit →
Line 18:		Line 18:
	*[[Melbourne/primary Ligase|T4 Ligase]]		*[[Melbourne/primary Ligase|T4 Ligase]]
	*[[Melbourne/primary Ligase buffer|T4 Ligase buffer]]		*[[Melbourne/primary Ligase buffer|T4 Ligase buffer]]
-	*[[Melbourne/primary competant DH5a|DH5a Competent cells]]
	*[[Melbourne/primary DNA marker|DNA ladder]]		*[[Melbourne/primary DNA marker|DNA ladder]]
	*[[Melbourne/primary phosphatase|phospatase]]		*[[Melbourne/primary phosphatase|phospatase]]

---

Revision as of 08:20, 28 September 2007

<Back to team home page> <Template>

Contents 1 Primary Reagents 1.1 Kits 1.2 Cells 1.3 Enzymes 1.4 Antibiotics 1.5 Chemicals 1.6 Other 2 Disposables 2.1 TUBES:

Primary Reagents

Kits

• IGEM 2007 kit
• Miniprep kit
• Gel clean up kit
• Site directed mutagenesis (stratagene)
  • Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb
  • Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb
  • QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb
• Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx

Cells

• pBluescriptKS+
• DH5a super competant cells

Enzymes

• Melbourne/primary Restriction enzymes
• T4 Ligase
• T4 Ligase buffer
• DNA ladder
• phospatase

Antibiotics

• Ampicilian
• Kanamycin

Chemicals

• Agar
• Agarose
• Ethidium Bromide
• EDTA
• Glycerol
• Loading dye for DNA gel
• NaCl
• TRIS

Other

• DNA Loading Dye
• ice
• milliQ water
• Distilled water

Disposables

• Parrafilm
• Tips (10, 200, 1000 ul)
• Gloves (Large , Med, Small)
• Scalpels
• Tube racks
• Freezer boxes

TUBES:

• Falcon: 10ml, 50ml
• microcentrifuge: 1.7ml
• PCR