From 2007.igem.org

<return to list of primary reagents> <team home page>

Contents 1 Physical data 2 Method 3 Lab Location 4 Store 5 Manufacturer:

Physical data

• Description:1Kb Plus DNA Ladder, 1ug/ml , 250ml , Store at -20 deg C
• apply 0.1ug of ladder per mm of lane width.
• Recomend BlueJuice loading buffer at 2x.

link to ladder jpg

Method

• Recieved ladder split into twelve 20uL alliquots and stored in ependorfs in Igem Freezer Box 1.
• To one alliquot 400uL of 1x Green Loading dye is added which turns it Blue.
• Place 20uL of this solution into the 10mm well. (1ug/well)(0.1ug/mm of well)

• link to user datasheet/manuals:
• Approximate cost: $0.00 AUD/mg

Lab Location

• Location
• Permision to use required? No

Store

• Available from Bio21 store? YES

Manufacturer:

• Manufacturer: Invitrogen
• Manufacturers Part No: Cat no. 10787-018
• Contact details for Manufacturer: www.invitrogen.com
• [http://www.invitrogen.com/content/sfs/manuals/10787018.pdf Manufacturers Manual]

• PhillipDodson 03:48, 1 July 2007 (EDT):Primary Reagent Template 1/July/2007