Melbourne/Primary Reagents & disposables
From 2007.igem.org
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*[[Melbourne/primary milliq|milliQ water]] | *[[Melbourne/primary milliq|milliQ water]] | ||
*[[Melbourne/primary distilled|Distilled water]] | *[[Melbourne/primary distilled|Distilled water]] | ||
| + | *[[Melbourne/primary Liquid N2|Liquid Nitrogen]] | ||
| + | *[[Melbourne/primary Solid CO2|Dry Ice]] | ||
| + | |||
==Disposables== | ==Disposables== | ||
*[[Melbourne/primary Parrafilm|Parrafilm]] | *[[Melbourne/primary Parrafilm|Parrafilm]] | ||
Revision as of 10:50, 29 September 2007
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Contents |
Primary Reagents
Kits
- IGEM 2007 kit
- Miniprep kit
- Gel clean up kit
- Site directed mutagenesis (stratagene)
- Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb
- Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb
- QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb
- Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx
Cells
Enzymes
- Melbourne/primary Restriction enzymes
- T4 Ligase
- T4 Ligase buffer 10X
- T4 Ligase buffer 2X
- DNA ladder
- phospatase
Antibiotics
Chemicals
Other
Disposables
- Parrafilm
- Tips (10, 200, 1000 ul)
- Gloves (Large , Med, Small)
- Scalpels
- Tube racks
- Freezer boxes
