Melbourne/Primary Reagents & disposables
From 2007.igem.org
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*[[Melbourne/primary IPTG|IPTG]] | *[[Melbourne/primary IPTG|IPTG]] | ||
*[[Melbourne/primary Xgal|Xgal]] | *[[Melbourne/primary Xgal|Xgal]] | ||
| + | *[[Melbourne/primary Bacto-tryptone|Bacto-tryptone]] | ||
| + | *[[Melbourne/primary Yeast Extract|Yeast Extract]] | ||
| + | *[[Melbourne/primary NaOH|NaOH]] | ||
===Other=== | ===Other=== | ||
Revision as of 11:02, 29 September 2007
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Contents |
Primary Reagents
Kits
- IGEM 2007 kit
- Miniprep kit
- Gel clean up kit
- Site directed mutagenesis (stratagene)
- Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb
- Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb
- QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb
- Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx
Cells
Enzymes
- Melbourne/primary Restriction enzymes
- T4 Ligase
- T4 Ligase buffer 10X
- T4 Ligase buffer 2X
- DNA ladder
- phospatase
Antibiotics
Chemicals
- Agar
- Agarose
- Ethidium Bromide
- EDTA
- Glycerol
- Loading dye for DNA gel
- NaCl
- TRIS
- IPTG
- Xgal
- Bacto-tryptone
- Yeast Extract
- NaOH
Other
Disposables
- Parrafilm
- Tips (10, 200, 1000 ul)
- Gloves (Large , Med, Small)
- Scalpels
- Tube racks
- Freezer boxes
