Paris/July 19
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| + | [[Paris/July 18|yesterday]] -- [[Paris/July 20|tomorrow]] <br> | ||
== FtsZ<sup>TS</sup> strain screening == | == FtsZ<sup>TS</sup> strain screening == | ||
We want to test these strains : | We want to test these strains : | ||
| Line 14: | Line 15: | ||
** One at 30°C | ** One at 30°C | ||
** One at 42°C | ** One at 42°C | ||
| - | Results : every strain grew at 30 and 42°C => | + | Results : every strain grew at 30 and 42°C => FAIL |
| + | |||
| + | == Overnight Bacterial cultures == | ||
| + | We launched several cultures : | ||
| + | |||
| + | *MG1655 -> to do transductions tomorrow (don't forget the DAP, David!) | ||
| + | *pJ23107_J61002 clones 3 & 4 in LB-Amp liquid; also striping clones 3-18 on LB-Amp Agar plates | ||
| + | *I0500_pSB2K3 clones 1 & 2 in LB-Kan liquid medium | ||
| + | *B0015_pSB1AK3 clones 3 & 4 in LB-Kan liquid medium | ||
| + | |||
| + | '''Tomorrow (20/07/07): perform transduction (MG1655), minipreps, glycerol stocks''' | ||
Latest revision as of 17:48, 7 October 2007
FtsZTS strain screening
We want to test these strains :
- 121.4
- 121.5
- 121.6
- 129
- 1.129
- DCR 14.1
- DCR 14.2
- DCR 14.3
I made glycerol stock of these strains, stored in the freezer. Test :
- Spread these strains on two plates (preheated before spreading) :
- One at 30°C
- One at 42°C
Results : every strain grew at 30 and 42°C => FAIL
Overnight Bacterial cultures
We launched several cultures :
- MG1655 -> to do transductions tomorrow (don't forget the DAP, David!)
- pJ23107_J61002 clones 3 & 4 in LB-Amp liquid; also striping clones 3-18 on LB-Amp Agar plates
- I0500_pSB2K3 clones 1 & 2 in LB-Kan liquid medium
- B0015_pSB1AK3 clones 3 & 4 in LB-Kan liquid medium
Tomorrow (20/07/07): perform transduction (MG1655), minipreps, glycerol stocks
