From 2007.igem.org

Revision as of 14:34, 14 September 2007 (view source) Davidpz (Talk | contribs) (→DapA Wanner deletion: pKD3/pKD4-DapA homologies PCR) ← Older edit		Latest revision as of 18:03, 7 October 2007 (view source) Nicolas C. (Talk | contribs)
(One intermediate revision not shown)
Line 1:		Line 1:
		+	[[Paris/September 11|yesterday]] -- [[Paris/September 13|tomorrow]] <br>
	== DapA Wanner deletion: pKD3/pKD4-DapA homologies PCR ==		== DapA Wanner deletion: pKD3/pKD4-DapA homologies PCR ==
Line 23:		Line 24:
	- 4°C: inf		- 4°C: inf
-	* '''Quantity:''' ng/µL <br>	+	* '''Quantity:''' 6ng/µL (pKD3) et 11ng/µL (pKD4) <br>
-	* '''Expected size:'''  bp	+	* '''Expected size:'''  1.1 kb (pKD3) / 1.6 kb (pKD4)
-		+
-	* After purification: <br>	+
-	2µL of purification product (2µL of ladder)	+

---

Latest revision as of 18:03, 7 October 2007

yesterday -- tomorrow

DapA Wanner deletion: pKD3/pKD4-DapA homologies PCR

• Material:
  

- pKD3/pKD4: 1 µL (30ng/60ng)
- Tampon: 10µL
- dNTP: 1µL (final 200µmol each)
- Taq: 0.5µL
- Water: 32.5µL
- Primer1: 33: 2.5µL (final 0.5µM)
- Primer2: 34: 2.5µL (final 0.5µM)

• PCR Program:
  

35 cycles:
- 95°C: 5min
- 95°C: 30s (X35)
- 55°C: 30s (X35)
- 72°C: 1min30 (X35)
- 72°C: 10 min
- 4°C: inf

• Quantity: 6ng/µL (pKD3) et 11ng/µL (pKD4)
  
• Expected size: 1.1 kb (pKD3) / 1.6 kb (pKD4)