Paris/Oligos

From 2007.igem.org

(Difference between revisions)
Line 9: Line 9:
|width=25%| Comments
|width=25%| Comments
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #ccffcc;" |1
+
| style="background: #ccffcc;" |O1
| Lox71-FtsZ-F
| Lox71-FtsZ-F
| TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATT ATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT
| TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATT ATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT
Line 15: Line 15:
|  
|  
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 2
+
| style="background: #ccffcc;" | O2
| FtsZ-R
| FtsZ-R
| TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT
| TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT
Line 21: Line 21:
|  
|  
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 3
+
| style="background: #ccffcc;" | O3
| Lox71-FtsA-F
| Lox71-FtsA-F
| TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACG AAGTTATTAATAATCAGGCACAGGCAGAACAACAATG
| TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACG AAGTTATTAATAATCAGGCACAGGCAGAACAACAATG
Line 27: Line 27:
|  
|  
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 4
+
| style="background: #ccffcc;" | O4
| DEcoR1-FtsZ-R
| DEcoR1-FtsZ-R
| CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC
| CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC
Line 33: Line 33:
|  
|  
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 5
+
| style="background: #ccffcc;" | O5
| DEcoR1-FtsZ-F  
| DEcoR1-FtsZ-F  
| TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA
| TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA
Line 39: Line 39:
|  
|  
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 6
+
| style="background: #ccffcc;" | O6
| Lox66-DapAColi-F
| Lox66-DapAColi-F
| TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAGTTTGCACAGAGGATGGCCCATGTT
| TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAGTTTGCACAGAGGATGGCCCATGTT
Line 46: Line 46:
   
   
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 7
+
| style="background: #ccffcc;" | O7
| DapAColi-R             
| DapAColi-R             
| TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC  
| TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC  
Line 53: Line 53:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" |8
+
| style="background: #ccffcc;" | O8
| Lox66-DapASubtilis-F     
| Lox66-DapASubtilis-F     
| TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAATCATGATGAGGTGAATAAAATGAA
| TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAATCATGATGAGGTGAATAAAATGAA
Line 60: Line 60:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 9
+
| style="background: #ccffcc;" | O9
|DapASubtilis-R  
|DapASubtilis-R  
| TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT
| TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT
Line 67: Line 67:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 10
+
| style="background: #ccffcc;" | O10
| Lox71-KmR-F           
| Lox71-KmR-F           
| TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACG AAGTTATCTGATCCTTCAACTCAGCAAAAGTTC
| TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACG AAGTTATCTGATCCTTCAACTCAGCAAAAGTTC
Line 74: Line 74:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 11
+
| style="background: #ccffcc;" | O11
| Lox66-KmR-R           
| Lox66-KmR-R           
| TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCA AGTTATTTATTAGAAAAACTCATCGAGCATCAAA  
| TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCA AGTTATTTATTAGAAAAACTCATCGAGCATCAAA  
Line 81: Line 81:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 12
+
| style="background: #ccffcc;" | O12
| DPst1-DGAT-F                     
| DPst1-DGAT-F                     
| AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT  
| AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT  
Line 88: Line 88:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 13
+
| style="background: #ccffcc;" | O13
| DPst1-DGAT-R           
| DPst1-DGAT-R           
| AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT  
| AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT  
Line 96: Line 96:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 14
+
| style="background: #ccffcc;" | O14
| Lox66-F       
| Lox66-F       
| AATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTATAC TAGTAGCGGCCGCTGCA
| AATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTATAC TAGTAGCGGCCGCTGCA
Line 103: Line 103:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 15
+
| style="background: #ccffcc;" | O15
| Lox66-R
| Lox66-R
| GCGGCCGCTACTAGTATACCGTTCGTATAATGTATGCTATACCAAGTTATCTCTAG AAGCGGCCGCG
| GCGGCCGCTACTAGTATACCGTTCGTATAATGTATGCTATACCAAGTTATCTCTAG AAGCGGCCGCG
Line 110: Line 110:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 16
+
| style="background: #ccffcc;" | O16
| Lox71-F
| Lox71-F
| AATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTACT AGTAGCGGCCGCTGCA
| AATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTACT AGTAGCGGCCGCTGCA
Line 117: Line 117:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 17
+
| style="background: #ccffcc;" | O17
| Lox71-R
| Lox71-R
| GCGGCCGCTACTAGTAATAACTTCGTATAATGTATCGTATACGAACGGTACTCTAG AAGCGGCCGCG
| GCGGCCGCTACTAGTAATAACTTCGTATAATGTATCGTATACGAACGGTACTCTAG AAGCGGCCGCG
Line 124: Line 124:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 18
+
| style="background: #ccffcc;" | O18
| VF2
| VF2
| tgccacctgacgtctaagaa
| tgccacctgacgtctaagaa
Line 131: Line 131:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 19
+
| style="background: #ccffcc;" | O19
| VR
| VR
| attaccgcctttgagtgagc
| attaccgcctttgagtgagc
Line 138: Line 138:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 20
+
| style="background: #ccffcc;" | O20
| RBS-DapAColi-F
| RBS-DapAColi-F
| TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGTT CACGGGAAGTATTGTCGCGA
| TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGTT CACGGGAAGTATTGTCGCGA
Line 145: Line 145:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 21
+
| style="background: #ccffcc;" | O21
| RBS-DapASubtilis-F
| RBS-DapASubtilis-F
| TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGAA TTTCGGAAATGTGTCTACAG
| TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGAA TTTCGGAAATGTGTCTACAG
Line 152: Line 152:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 22
+
| style="background: #ccffcc;" | O22
| SEQFtsA-1F
| SEQFtsA-1F
| GTCGTCCATACCGCGAAATC
| GTCGTCCATACCGCGAAATC
Line 159: Line 159:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 23
+
| style="background: #ccffcc;" | O23
| SEQFtsA-2F
| SEQFtsA-2F
| CGTGGAAGTGCCGAGCGTAG
| CGTGGAAGTGCCGAGCGTAG
Line 166: Line 166:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 24
+
| style="background: #ccffcc;" | O24
| SEQFtsZ-1F
| SEQFtsZ-1F
| GGTATCGCTGAACTGATTAC
| GGTATCGCTGAACTGATTAC
Line 173: Line 173:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 25
+
| style="background: #ccffcc;" | O25
| SEQDapASub-1F
| SEQDapASub-1F
| TTAAAGCAATTGCGGCAGAG
| TTAAAGCAATTGCGGCAGAG
Line 180: Line 180:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 26
+
| style="background: #ccffcc;" | O26
| SEQDapAColi-1F
| SEQDapAColi-1F
| CAATCGTCCGTCGCAAGAAG
| CAATCGTCCGTCGCAAGAAG
Line 187: Line 187:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 27
+
| style="background: #ccffcc;" | O27
| ForDGAT1
| ForDGAT1
| ATCCACGCTATGCGCCCATTAC
| ATCCACGCTATGCGCCCATTAC
Line 194: Line 194:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 28
+
| style="background: #ccffcc;" | O28
| RevDGAT1
| RevDGAT1
| GGGCTAATTTAGCCCTTTAGTT
| GGGCTAATTTAGCCCTTTAGTT
Line 201: Line 201:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 29
+
| style="background: #ccffcc;" | O29
| ForDGAT_BB1
| ForDGAT_BB1
| AAAGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAAATCCACGCTATGCGCCCATTAC
| AAAGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAAATCCACGCTATGCGCCCATTAC
Line 208: Line 208:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 30
+
| style="background: #ccffcc;" | O30
| RevDGAT_BB1
| RevDGAT_BB1
| CTGCAGCGGCCGCTACTAGTAGGGCTAATTTAGCCCTTTAGTT
| CTGCAGCGGCCGCTACTAGTAGGGCTAATTTAGCCCTTTAGTT
Line 215: Line 215:
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 31
+
| style="background: #ccffcc;" | O31
| DapAp-F
| DapAp-F
| aattcgcggccgcttctagagAACAATCAGAACGGTTCTGTCTGCTTGCTTTT AATGCCATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGATGGta
| aattcgcggccgcttctagagAACAATCAGAACGGTTCTGTCTGCTTGCTTTT AATGCCATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGATGGta
Line 221: Line 221:
|  
|  
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 32
+
| style="background: #ccffcc;" | O32
| DapAp-R
| DapAp-R
| ctagtaCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCA TTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTctctagaagcggccgcg
| ctagtaCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCA TTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTctctagaagcggccgcg
Line 227: Line 227:
|  
|  
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 33
+
| style="background: #ccffcc;" | O33
| dDapA-F
| dDapA-F
| CATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGAT GGCCCATGATTCCGGGGATCCGTCGACC
| CATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGAT GGCCCATGATTCCGGGGATCCGTCGACC
Line 233: Line 233:
| [[Paris/July 27| more here]]
| [[Paris/July 27| more here]]
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 34
+
| style="background: #ccffcc;" | O34
| dDapA-R
| dDapA-R
| AAGCCATCAAATCTCCCTAAACTTTACAGCAAACCGGCATGC TTAAGCGCTGTAGGCTGGAGCTGCTTCG
| AAGCCATCAAATCTCCCTAAACTTTACAGCAAACCGGCATGC TTAAGCGCTGTAGGCTGGAGCTGCTTCG
Line 239: Line 239:
| [[Paris/July 27| more here]]
| [[Paris/July 27| more here]]
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 35
+
| style="background: #ccffcc;" | O35
| dFtsZ-F
| dFtsZ-F
| GACGATGATTACGGCCTCAGGCGACAGGCACAAATCGGAGAGA AACTATGATTCCGGGGATCCGTCGACC
| GACGATGATTACGGCCTCAGGCGACAGGCACAAATCGGAGAGA AACTATGATTCCGGGGATCCGTCGACC
Line 245: Line 245:
| [[Paris/July 27| more here]]
| [[Paris/July 27| more here]]
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 36
+
| style="background: #ccffcc;" | O36
| dFtsZ-R
| dFtsZ-R
| GAAACCCAAATTCCAGTCAATTCTTAATCAGCTTGCTTACGCA GGAATGCTGTAGGCTGGAGCTGCTTCG
| GAAACCCAAATTCCAGTCAATTCTTAATCAGCTTGCTTACGCA GGAATGCTGTAGGCTGGAGCTGCTTCG
Line 251: Line 251:
| [[Paris/July 27| more here]]
| [[Paris/July 27| more here]]
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 37
+
| style="background: #ccffcc;" | O37
| dDapAverif-F
| dDapAverif-F
| ACAATCAGAACGGTTCTGTCTG
| ACAATCAGAACGGTTCTGTCTG
Line 257: Line 257:
|  
|  
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 38
+
| style="background: #ccffcc;" | O38
| dDapAverif-R
| dDapAverif-R
| ATAGCGTGAGTCAGAACTACAG
| ATAGCGTGAGTCAGAACTACAG
Line 263: Line 263:
|  
|  
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 39
+
| style="background: #ccffcc;" | O39
| dFtsZverif-R
| dFtsZverif-R
| TCGCCAAATTACCTATCCAACC
| TCGCCAAATTACCTATCCAACC
Line 269: Line 269:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 40
+
| style="background: #ccffcc;" | O40
| SEQ-mRFP-R
| SEQ-mRFP-R
| CGGACGACCTTCACCTTCAC
| CGGACGACCTTCACCTTCAC
Line 275: Line 275:
|  
|  
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 41
+
| style="background: #ccffcc;" | O41
| M13(-20)-F
| M13(-20)-F
|GTAAAACGACGGCCAG  
|GTAAAACGACGGCCAG  
Line 281: Line 281:
|  
|  
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 42
+
| style="background: #ccffcc;" | O42
| M13-R
| M13-R
| CAGGAAACAGCTTATGAC
| CAGGAAACAGCTTATGAC
Line 287: Line 287:
|  
|  
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 43
+
| style="background: #ccffcc;" | O43
| Bfr-FtsK-F
| Bfr-FtsK-F
| TTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAATGCCACCTGACGTCTAAGAA
| TTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAATGCCACCTGACGTCTAAGAA
Line 293: Line 293:
|  
|  
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 44
+
| style="background: #ccffcc;" | O44
| Bfr-FtsK-R
| Bfr-FtsK-R
| CCAGGTTACCCCAGTACTGTTGCTGTATGGATTAACAGGAGTGTAATCAAATTACCGCCTTTGAGTGAGC
| CCAGGTTACCCCAGTACTGTTGCTGTATGGATTAACAGGAGTGTAATCAAATTACCGCCTTTGAGTGAGC
Line 299: Line 299:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 45
+
| style="background: #ccffcc;" | O45
| Aft-FtsK-F
| Aft-FtsK-F
| AGGGGCACAACGGTAATCGTGAAGTGCTGGCCCCACCGCCGTTTGACTAATGCCACCTGACGTCTAAGAA
| AGGGGCACAACGGTAATCGTGAAGTGCTGGCCCCACCGCCGTTTGACTAATGCCACCTGACGTCTAAGAA
Line 305: Line 305:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | 46
+
| style="background: #ccffcc;" | O46
| Aft-FtsK-R
| Aft-FtsK-R
| GAAAATACTGAATTTTCAGTTGATAGAGTGCCGGATGCGACGCTACCGCGATTACCGCCTTTGAGTGAGC
| GAAAATACTGAATTTTCAGTTGATAGAGTGCCGGATGCGACGCTACCGCGATTACCGCCTTTGAGTGAGC

Revision as of 20:45, 9 October 2007

Table of Oligos
Number Name Sequence Length Comments
O1 Lox71-FtsZ-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATT ATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT 89
O2 FtsZ-R TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT 49
O3 Lox71-FtsA-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACG AAGTTATTAATAATCAGGCACAGGCAGAACAACAATG 89
O4 DEcoR1-FtsZ-R CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC 40
O5 DEcoR1-FtsZ-F TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA 40
O6 Lox66-DapAColi-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAGTTTGCACAGAGGATGGCCCATGTT 84
O7 DapAColi-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC 52
O8 Lox66-DapASubtilis-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAATCATGATGAGGTGAATAAAATGAA 84
O9 DapASubtilis-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT 52
O10 Lox71-KmR-F TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACG AAGTTATCTGATCCTTCAACTCAGCAAAAGTTC 85
O11 Lox66-KmR-R TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCA AGTTATTTATTAGAAAAACTCATCGAGCATCAAA 86
O12 DPst1-DGAT-F AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT 40
O13 DPst1-DGAT-R AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT 40


O14 Lox66-F AATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTATAC TAGTAGCGGCCGCTGCA
O15 Lox66-R GCGGCCGCTACTAGTATACCGTTCGTATAATGTATGCTATACCAAGTTATCTCTAG AAGCGGCCGCG
O16 Lox71-F AATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTACT AGTAGCGGCCGCTGCA
O17 Lox71-R GCGGCCGCTACTAGTAATAACTTCGTATAATGTATCGTATACGAACGGTACTCTAG AAGCGGCCGCG
O18 VF2 tgccacctgacgtctaagaa 20 Forward Primer to Biobrick vectors (VF2) BBa_G00100
O19 VR attaccgcctttgagtgagc 20 Reverse Primer to Biobrick Vectors (VR) BBa_G00101
O20 RBS-DapAColi-F TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGTT CACGGGAAGTATTGTCGCGA 71 Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Coli) from ATG
O21 RBS-DapASubtilis-F TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGAA TTTCGGAAATGTGTCTACAG 71 Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Subtilis) from ATG
O22 SEQFtsA-1F GTCGTCCATACCGCGAAATC 20
O23 SEQFtsA-2F CGTGGAAGTGCCGAGCGTAG 20
O24 SEQFtsZ-1F GGTATCGCTGAACTGATTAC 20
O25 SEQDapASub-1F TTAAAGCAATTGCGGCAGAG 20
O26 SEQDapAColi-1F CAATCGTCCGTCGCAAGAAG 20
O27 ForDGAT1 ATCCACGCTATGCGCCCATTAC 22 Tm = 60°C
O28 RevDGAT1 GGGCTAATTTAGCCCTTTAGTT 22 Tm = 50°C
O29 ForDGAT_BB1 AAAGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAAATCCACGCTATGCGCCCATTAC 62 Tm = 60/83°C
O30 RevDGAT_BB1 CTGCAGCGGCCGCTACTAGTAGGGCTAATTTAGCCCTTTAGTT 43 Tm = 50/74°C
O31 DapAp-F aattcgcggccgcttctagagAACAATCAGAACGGTTCTGTCTGCTTGCTTTT AATGCCATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGATGGta 104
O32 DapAp-R ctagtaCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCA TTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTctctagaagcggccgcg 104
O33 dDapA-F CATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGAT GGCCCATGATTCCGGGGATCCGTCGACC 70 more here
O34 dDapA-R AAGCCATCAAATCTCCCTAAACTTTACAGCAAACCGGCATGC TTAAGCGCTGTAGGCTGGAGCTGCTTCG 70 more here
O35 dFtsZ-F GACGATGATTACGGCCTCAGGCGACAGGCACAAATCGGAGAGA AACTATGATTCCGGGGATCCGTCGACC 70 more here
O36 dFtsZ-R GAAACCCAAATTCCAGTCAATTCTTAATCAGCTTGCTTACGCA GGAATGCTGTAGGCTGGAGCTGCTTCG 70 more here
O37 dDapAverif-F ACAATCAGAACGGTTCTGTCTG 22
O38 dDapAverif-R ATAGCGTGAGTCAGAACTACAG 22
O39 dFtsZverif-R TCGCCAAATTACCTATCCAACC 22
O40 SEQ-mRFP-R CGGACGACCTTCACCTTCAC
O41 M13(-20)-F GTAAAACGACGGCCAG
O42 M13-R CAGGAAACAGCTTATGAC
O43 Bfr-FtsK-F TTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAATGCCACCTGACGTCTAAGAA 70
O44 Bfr-FtsK-R CCAGGTTACCCCAGTACTGTTGCTGTATGGATTAACAGGAGTGTAATCAAATTACCGCCTTTGAGTGAGC 70
O45 Aft-FtsK-F AGGGGCACAACGGTAATCGTGAAGTGCTGGCCCCACCGCCGTTTGACTAATGCCACCTGACGTCTAAGAA 70
O46 Aft-FtsK-R GAAAATACTGAATTTTCAGTTGATAGAGTGCCGGATGCGACGCTACCGCGATTACCGCCTTTGAGTGAGC 70
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