BerkiGEM2007-SamanthaConstructionFile5
From 2007.igem.org
(Difference between revisions)
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| - | PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI) | + | PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI) <br> |
| - | Sub into pBca9145-Bca1089 (BglII/XhoI,2063+696, L) | + | Sub into pBca9145-Bca1089 (BglII/XhoI,2063+696, L) <br> |
| - | Product is pBca9145-I716209 | + | Product is pBca9145-I716209 <br> |
---------------------------- | ---------------------------- | ||
| - | sil04F Forward BglII biobrick of BamHI | + | sil04F Forward BglII biobrick of BamHI <br> ccaaaAGATCTgaagtagaaaaagagtttattactga <br> |
| - | sil04R Reverse XholI/BamHI biobrick of BamHI | + | sil04R Reverse XholI/BamHI biobrick of BamHI <br> gctagCTCGAGttaGGATCCctatttgttttcaactttatc <br> |
Revision as of 22:57, 4 June 2007
Biobricking of BamHI endonuclease without start codon
PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI,2063+696, L)
Product is pBca9145-I716209
sil04F Forward BglII biobrick of BamHI
ccaaaAGATCTgaagtagaaaaagagtttattactga
sil04R Reverse XholI/BamHI biobrick of BamHI
gctagCTCGAGttaGGATCCctatttgttttcaactttatc
