Paris/Constructs

From 2007.igem.org

< Paris(Difference between revisions)
 
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== Construct : Forward construction ==
<html>
<html>
<object classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" codebase="http://download.macromedia.com/pub/shockwave/cabs/flash/swflash.cab#version=6,0,29,0" width="800" height="600">
<object classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" codebase="http://download.macromedia.com/pub/shockwave/cabs/flash/swflash.cab#version=6,0,29,0" width="800" height="600">
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<param name="movie" value="http://poupitoupou.free.fr/test.swf">
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<param name="movie" value="http://poupitoupou.free.fr/Construct_FW.swf">
<param name="quality" value="high">
<param name="quality" value="high">
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<embed src="http://poupitoupou.free.fr/test.swf" quality="high" pluginspage="http://www.macromedia.com/go/getflashplayer" type="application/x-shockwave-flash" width="800" height="600"></embed></object>
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<embed src="http://poupitoupou.free.fr/Construct_FW.swf" quality="high" pluginspage="http://www.macromedia.com/go/getflashplayer" type="application/x-shockwave-flash" width="500" height="750"></embed></object>
</html>
</html>
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Voici un exemple de présentation de construction de biologie moléculaire. La case info sert à renvoyer vers les pages où les manips ont étés faites pour chaque partie, ou pour tout autre information utile à la "part".
 
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[http://poupitoupou.free.fr/test.swf test]
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== Construct : Backward construct ==
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{|
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=== Intermediate construct ===
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<br>
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<html>
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|align="center"|{{Paris_Object|
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<object classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" codebase="http://download.macromedia.com/pub/shockwave/cabs/flash/swflash.cab#version=6,0,29,0" width="800" height="600">
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|Nom = Lox71
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<param name="movie" value="http://poupitoupou.free.fr/Construct_Intermediate.swf">
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|Ref = O16+O17
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<param name="quality" value="high">
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|Img = Paris_O16_O17.png
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<embed src="http://poupitoupou.free.fr/Construct_Intermediate.swf" quality="high" pluginspage="http://www.macromedia.com/go/getflashplayer" type="application/x-shockwave-flash" width="400" height="500"></embed></object>
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|Info= [[Paris/Oligos|Sequence]]  [[Paris/July_24#Annealing_of_Lox66_and_Lox71|Annealing]]
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</html>
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}}
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===  Backward construct with DapA E.Coli ===
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|align="center"|{{Paris_Object|
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<br>
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|Nom = pSB1A2
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<html>
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|Ref = D22
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<object classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" codebase="http://download.macromedia.com/pub/shockwave/cabs/flash/swflash.cab#version=6,0,29,0" width="800" height="600">
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|Img = Paris_D22.png
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<param name="movie" value="http://poupitoupou.free.fr/Construct_BW_DapE.swf">
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|Info= [[Paris/July_23#Digestion_reactions|Digest]]
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<param name="quality" value="high">
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}}
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<embed src="http://poupitoupou.free.fr/Construct_BW_DapE.swf" quality="high" pluginspage="http://www.macromedia.com/go/getflashplayer" type="application/x-shockwave-flash" width="450" height="350"></embed></object>
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</html>
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|-
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|align="center"|{{Paris_Object|
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===  Backward construct with DapA Subtilis ===
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|Nom = BBa_E0840
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<br>
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|Ref = MP8
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<html>
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|Img = Paris_MP8.png
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<object classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" codebase="http://download.macromedia.com/pub/shockwave/cabs/flash/swflash.cab#version=6,0,29,0" width="800" height="600">
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|Info= [[Paris/July_18|Miniprep]]
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<param name="movie" value="http://poupitoupou.free.fr/Construct_BW_DapS.swf">
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}}
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<param name="quality" value="high">
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|align="center"|[[Image:Paris_RArrow.png]]
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<embed src="http://poupitoupou.free.fr/Construct_BW_DapS.swf" quality="high" pluginspage="http://www.macromedia.com/go/getflashplayer" type="application/x-shockwave-flash" width="600" height="550"></embed></object>
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|align="center"|{{Paris_Object|
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</html>
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|Nom = pSB1A2-Lox71
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|Ref = L10
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== Construct : recombinaison rate measurement ==
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|Img = Paris_L10.png
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<br>
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|Info= [[Paris/July_24|Ligation]]
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<html>
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}}
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<object classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" codebase="http://download.macromedia.com/pub/shockwave/cabs/flash/swflash.cab#version=6,0,29,0" width="800" height="600">
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|align="center"|[[Image:Paris_LArrow.png]]
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<param name="movie" value="http://poupitoupou.free.fr/Construct_MRR.swf">
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<param name="quality" value="high">
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<embed src="http://poupitoupou.free.fr/Construct_MRR.swf" quality="high" pluginspage="http://www.macromedia.com/go/getflashplayer" type="application/x-shockwave-flash" width="500" height="600"></embed></object>
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|align="center"|[[Image:Paris_DArrow.png]]
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</html>
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|align="center"|[[Image:Paris_DArrow.png]]
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|align="center"|{{Paris_Object|
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|Nom = GFP Tri-Part
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|Ref = D26
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|Img = Paris_D26.png
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|Info= [[Paris/July_23|Digest]]
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}}
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|align="center"|{{Paris_Object|
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|Nom = pSB1A2-Lox71
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|Ref = D35
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|Img = Paris_D35.png
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|Info= [[Paris/July_30|Digest]]
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}}
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|-
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|align="center"|[[Image:Paris_RArrow.png]]
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|align="center"|{{Paris_Object|
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|Nom = lox71 - GFP Tri-Part
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|Ref = L12
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|Img = Paris_L12.png
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|Info= [[Paris/July_30|Ligation]]
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}}
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|align="center"|[[Image:Paris_LArrow.png]]
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|align="center"|{{Paris_Object|
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|Nom = pTet>>mRFP
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|Ref = MP13
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|Img = Paris_MP13.png
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|Info= [[Paris/August_14|Miniprep]]
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}}
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|align="center"|[[Image:Paris_DArrow.png]]
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|align="center"|[[Image:Paris_DArrow.png]]
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|align="center"|{{Paris_Object|
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|Nom = lox71 - GFP Tri-Part
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|Ref = D49
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|Img = Paris_D49.png
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|Info= [[Paris/August_15|Digest]]
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}}
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|align="center"|{{Paris_Object|
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|Nom = pTet>>
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|Ref = D50
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|Img = Paris_D50.png
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|Info= [[Paris/August_15|Digest]]
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}}
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|align="center"| [[Image:Paris_RArrow.png]]
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|align="center"|{{Paris_Object|
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|Nom = pTet>>lox71 - GFP Tri-Part
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|Ref = L34
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|Img = Paris_L34.png
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|Info= [[Paris/August_20|Ligation]] [[Paris/August_22|Miniprep]]
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}}
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|align="center"| [[Image:Paris_LArrow.png]]
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|}
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Latest revision as of 08:25, 19 October 2007

Contents

Construct : Forward construction

Construct : Backward construct

Intermediate construct


Backward construct with DapA E.Coli


Backward construct with DapA Subtilis


Construct : recombinaison rate measurement


Retrieved from "http://2007.igem.org/wiki/index.php/Paris/Constructs"