From 2007.igem.org

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-	NOT DONE YET...found an ALWNI site in Cre, use BglI instead	+
	'''[rbs][Cre]''' <br>		'''[rbs][Cre]''' <br>

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Revision as of 00:21, 8 June 2007

[rbs][Cre]
rbs variants Bca1090
2 Different Cre variants (212 and 213)

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Digest pBca9145-Bca1090 (BamHI/BglI/AlwNI, 942+596+553bp, Large)
Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716217.

Digest pBca9145-Bca1090 (BamHI/BglI, 942+596+553bp, Large)
Digest pBca9145-I716213 (BglII/BglI, 2187+923bp, Small)
Product is pBca9145-I716221.