NYMU Taipei/Protocols2

From 2007.igem.org

(Difference between revisions)
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     <li><font size="2">Quantitation of&nbsp; Nucleic acid concentration</font>
     <li><font size="2">Quantitation of&nbsp; Nucleic acid concentration</font>
     <ul>
     <ul>
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         <li><font size="2"><a href="http://en.wikipedia.org/wiki/Quantification_of_nucleic_acids">Wikipedia</a></font></li>
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         <li><font size="2">[[Wikipedia]]</font></li>
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         <li><font size="2"><a href="http://www.piercenet.com/Objects/View.cfm?type=Page&amp;ID=0A0A7BB1-7582-4611-A116-6F583EC01666">Pierce</a></font></li>
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         <li><font size="2">[[Pierce]]</font></li>
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         <li><font size="2"><a href="http://mcbl.iisc.ernet.in/Welcome%20to%20MCBL/Faculty/Parag/microarray%20workshop%20details/quantitation.html">India</a></font></li>
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         <li><font size="2">[[India]]</font></li>
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         <li><font size="2"><a href="http://www.kreatech.com/pages/Focus-Areas/*-Hybridization-___-Labeling-protocols/Small-RNA-Labeling-protocol/">Kreatech Biotechnology</a>&nbsp;</font></li>
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         <li><font size="2">[[Kreatech Biotechnology]]&nbsp;</font></li>
     </ul>
     </ul>
     </li>
     </li>
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     <li><font face="Arial" size="2"><span lang="EN-US" style="font-size: 9.5pt; font-family: Arial"><a href="http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/setting_up_reaction.asp">Setting Up a Restriction Endonuclease Reaction</a></span></font><font size="2"> </font></li>
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     <li><font face="Arial" size="2"><span lang="EN-US" style="font-size: 9.5pt; font-family: Arial">[[ </font></li>
     <li><font size="2">DNA Analysis through Gel Electrophoresis</font></li>
     <li><font size="2">DNA Analysis through Gel Electrophoresis</font></li>
     <li><font size="2">Ligate blunt or cohesive ends in 5 minutes at room temperature</font>
     <li><font size="2">Ligate blunt or cohesive ends in 5 minutes at room temperature</font>
     <ul>
     <ul>
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         <li><font size="2"><a href="http://www.neb.com/nebecomm/products/productM2200.asp">Quick Ligation Kit&nbsp;(NEB)</a></font></li>
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         <li><font size="2">[[Quick Ligation Kit&nbsp;(NEB)]]</font></li>
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         <li><font size="2"><a href="http://www.ubi.ca/cart/index.php/cPath/27_163_165">Fast Ligation Kit (UBI)</a></font></li>
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         <li><font size="2">[[Fast Ligation Kit (UBI)]]</font></li>
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         <li><font size="2"><a href="http://www.epibio.com/item.asp?ID=296">Fast-Link DNA Ligation Kit (EPICENTRE Biotechnologies)</a></font></li>
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         <li><font size="2">[[Fast-Link DNA Ligation Kit (EPICENTRE Biotechnologies)]]</font></li>
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         <li><font size="2"><a href="http://www.fermentas.com/catalog/kits/kitrapidligation.htm">Rapid Ligation Kit (Fermentas)</a></font></li>
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         <li><font size="2">[[Rapid Ligation Kit (Fermentas)]]</font></li>
         <li><font size="2">All of these are just normal ligase buffers with PEG8000 at a 10-12% concentration. The NEB catalogue gives the original reference.&nbsp;<br />
         <li><font size="2">All of these are just normal ligase buffers with PEG8000 at a 10-12% concentration. The NEB catalogue gives the original reference.&nbsp;<br />
         Use of crowding agents like PEG allow 5-minute sticky end ligations.</font></li>
         Use of crowding agents like PEG allow 5-minute sticky end ligations.</font></li>
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         <li><font size="2">Vector sequences </font>
         <li><font size="2">Vector sequences </font>
         <ul>
         <ul>
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             <li><a href="http://seq.yeastgenome.org/vectordb/"><font size="2">VectorDB - Molecular Biology Vector Sequence Database</font></a><font size="2"> </font></li>
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             <li>[[ </font></li>
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             <li><a href="http://www.shigen.nig.ac.jp/cvector/cvector.html"><font size="2">The cloning vector collection</font></a><font size="2"> </font></li>
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             <li>[[ </font></li>
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             <li><a href="http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/dna_sequences_maps.asp"><font size="2">New England Biolabs (NEB) DNA Sequences and Maps</font></a><font size="2"> </font></li>
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             <li>[[ </font></li>
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             <li><a href="http://www.promega.com/vectors/cloning_vectors.htm"><font size="2">Promega Cloning Vectors</font></a><font size="2"> </font></li>
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             <li>[[ </font></li>
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             <li><a href="http://www.emdbiosciences.com/Products/BrowseProductsByCategory.asp?catid=1628"><font size="2">Novagen Vector Maps</font></a><font size="2"> </font></li>
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             <li>[[ </font></li>
         </ul>
         </ul>
         </li>
         </li>
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         <li><a href="http://wishart.biology.ualberta.ca/PlasMapper/"><font size="2">PlasMapper Version 2.0 - automatically generates and annotates plasmid maps</font></a><font size="2"> </font></li>
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         <li>[[ </font></li>
     </ul>
     </ul>
     </li>
     </li>
     <li><font size="2">[[Transformation]] </font></li>
     <li><font size="2">[[Transformation]] </font></li>
     <li><font size="2">[[Production of heterologous proteins in Escherichia coli]] </font></li>
     <li><font size="2">[[Production of heterologous proteins in Escherichia coli]] </font></li>
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     <li><a href="http://openwetware.org/wiki/Endy:Preparing_Antibiotic_Stocks"><font size="2">Antibiotics</font></a><font size="2"> </font></li>
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     <li>[[ </font></li>
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     <li><a href="http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication"><font face="Arial" size="2">Part fabrication</font></a><font size="2"> (</font><a href="http://openwetware.org/wiki/Enzyme_selection_for_BioBricks_digest"><font size="2">Enzyme selection for BioBricks digest</font></a><font size="2">!!) </font></li>
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     <li>[[!!) </font></li>
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     <li><a href="http://openwetware.org/wiki/BioBricks_construction_tutorial"><font size="2">BioBricks construction tutorial</font></a><font size="2"> </font></li>
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     <li>[[ </font></li>
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     <li><a href="http://openwetware.org/wiki/Silver:_BB_Strategy"><font size="2">Fusion protein</font></a><font size="2"> </font></li>
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     <li>[[ </font></li>
     <li><font size="2">Glucose assay </font>
     <li><font size="2">Glucose assay </font>
     <ul>
     <ul>
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         <li><font color="#000000" size="2"><a href="http://en.wikipedia.org/wiki/Blood_sugar">Blood sugar (from Wikipedia)</a></font><font size="2"> </font></li>
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         <li><font color="#000000" size="2">[[ </font></li>
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         <li><a href="http://www.genengnews.com/articles/chtitem.aspx?tid=1624&amp;chid=3"><font size="2">Glucose Measurement for Cell Culture</font></a><font size="2"> </font></li>
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         <li>[[ </font></li>
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         <li><a href="http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Metabolomics/Product_Highlights/Enzymatic_Kits.html"><font size="2">Glucose Assay Kits from SigmaAldrich</font></a><font size="2"> </font></li>
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         <li>[[ </font></li>
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         <li><a href="http://www.bioassaysys.com/Order.html"><font size="2">Glucose Assay Kit from QuantiChrom</font></a><font size="2"> </font></li>
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         <li>[[ </font></li>
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         <li><a href="http://www.rpbiotech.com/Biochem_diag_kits/Glucose_Diagnostic_Kit.htm"><font size="2">Biochemical glucose kit from rpbiotech</font></a><font size="2"> </font></li>
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         <li>[[ </font></li>
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         <li><a href="http://www.anilinepharma.com/products.php?catg=ds&amp;id=1"><font size="2">Glucose Meter from AnilinePharma</font></a><font size="2"> </font></li>
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         <li>[[ </font></li>
     </ul>
     </ul>
     </li>
     </li>
     <li><font size="2">Mammalian Gene Collection (MGC) Full-length cDNA clones </font>
     <li><font size="2">Mammalian Gene Collection (MGC) Full-length cDNA clones </font>
     <ul>
     <ul>
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         <li><a href="http://mgc.nci.nih.gov/"><font size="2">at National Cancer Institute of NIH</font></a><font size="2"> </font></li>
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         <li>[[ </font></li>
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         <li><a href="http://genome.ym.edu.tw/libres/MGC_clones/index.htm"><font size="2">at Genome Research Center of NYMU</font></a><font size="2"> </font></li>
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         <li>[[ </font></li>
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         <li><a href="http://www.ncbi.nlm.nih.gov/FLC/getmgc.cgi"><font size="2">NCBI MGC retrieval</font></a><font size="2"> </font></li>
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         <li>[[ </font></li>
     </ul>
     </ul>
     </li>
     </li>
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<h3>Dry-Lab Protocols</h3>
<h3>Dry-Lab Protocols</h3>
<ul>
<ul>
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     <li><a href="http://serialbasics.free.fr/Serial_Cloner.html">serial cloner</a></li>
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     <li>[[serial cloner]]</li>
     <li>Protein Fusion Design
     <li>Protein Fusion Design
     <ul>
     <ul>
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         <ul>
         <ul>
             <li>[[Properties of TAT Signal Peptides]]</li>
             <li>[[Properties of TAT Signal Peptides]]</li>
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             <li><a href="http://www.jic.bbsrc.ac.uk/staff/tracy-palmer/signals.htm">A link to a list of Tat signal sequences provided by Tracy Palmer</a></li>
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             <li>[[A link to a list of Tat signal sequences provided by Tracy Palmer]]</li>
             <li>[[29 putative signal peptides]]&nbsp;</li>
             <li>[[29 putative signal peptides]]&nbsp;</li>
             <li>[[Selected TAT signal peptides]]</li>
             <li>[[Selected TAT signal peptides]]</li>
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<h3>General references</h3>
<h3>General references</h3>
<ul>
<ul>
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     <li><a href="http://www.neb.com/nebecomm/tech_reference/default.asp">New England Biolab (NEB) technical references</a></li>
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     <li>[[New England Biolab (NEB) technical references]]</li>
</ul>
</ul>
[https://2007.igem.org/NYMU_Taipei/Welcome Back]
[https://2007.igem.org/NYMU_Taipei/Welcome Back]

Revision as of 16:37, 19 October 2007

Wet-Lab Protocols

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