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| | |rbsdapAsubtilis extracted for use as a BI | | |rbsdapAsubtilis extracted for use as a BI |
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| - | More digestions that need to be performed
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| - | |- style="background: #ccccff;"
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| - | ! colspan="5" style="background: #ccccff;" | Digestion Products
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| - | |- style="background: #ccccff; text-align: center;"
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| - | |width=5%| Number
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| - | |width=24%| Product Name
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| - | |width=24%| Matrix Name
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| - | |width=5%| Enzyme 1
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| - | |width=5%| Enzyme 2
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| - | |width=5%| Size
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| - | |width=34%| Description
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| - | |width=1%|
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| - | |width=1%|
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| - | |width=1%|
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| - | |- style="background: #cccccc;"
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| - | | style="background: #ccffcc;" |D76
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| - | |DGAT BB
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| - | |P14(19/10/07)
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| - | |EcoRI
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| - | |PstI
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| - | |DGAT (delta PstI)
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| - | |- style="background: #cccccc;"
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| - | | style="background: #ccffcc;" |D79
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| - | |DGAT BI
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| - | |P14(19/10/07)
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| - | |XbaI
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| - | |PstI
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| - | |DGAT(delta PstI)PCR product for cloming as a BB in D47
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| - | |- style="background: #cccccc;"
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| - | | style="background: #ccffcc;" |D80
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| - | |PacI[ptet>>lox71-gfpT-lox66-mrfp]SmaI
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| - | |P15
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| - | |PacI
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| - | |SmaI
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| - | |
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| - | |ptet>>lox71-gfpT-lox66-mrfp P15 PCR product digested by PacI-SmaI for cloning into pY-2C-IntC
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| - | |- style="background: #cccccc;"
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| - | | style="background: #ccffcc;" |D81
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| - | |PacI[pY-2C-IntC]SmaI
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| - | |pY-2C-IntC plasmid
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| - | |PacI
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| - | |SmaI
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| - | |pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning
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| - | |- style="background: #cccccc;"
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| - | | style="background: #ccffcc;" |D82
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| - | |frt1-CmR-frt2 FI
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| - | |P16
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| - | |EcoRI
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| - | |SpeI
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| - | |frt1-CmR-frt2 PCR product digested for use as a FI
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| - | |- style="background: #cccccc;"
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| - | | style="background: #ccffcc;" |D83
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| - | |frt1-CmR-frt2 BI
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| - | |P16
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| - | |XbaI
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| - | |PstI
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| - | |
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| - | |frt1-CmR-frt2 PCR product digested for use as a BI
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| - | |- style="background: #cccccc;"
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| - | | style="background: #ccffcc;" |D84
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| - | |frt1-CmR-frt2 BBi
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| - | |P16
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| - | |EcoRI
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| - | |PstI
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| - | |
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| - | |frt1-CmR-frt2 PCR product digested for cloning as a BB
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| - | |
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| - | |
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| - | |- style="background: #cccccc;"
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| - | | style="background: #ccffcc;" |D85
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| - | |frt1-CmR-frt2-T1T2 FI
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| - | |P17
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| - | |EcoRI
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| - | |SpeI
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| - | |
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| - | |frt1-CmR-frt2-T1T2 PCR product digested for use as a FI
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| - | |
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| - | |
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| - | |
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| - | |- style="background: #cccccc;"
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| - | | style="background: #ccffcc;" |D86
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| - | |frt1-CmR-frt2-T1T2 BI
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| - | |P17
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| - | |XbaI-
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| - | |PstI
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| - | |
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| - | |frt1-CmR-frt2-T1T2 PCR product digested for use as a BI
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| - | |
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| - | |- style="background: #cccccc;"
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| - | | style="background: #ccffcc;" |D87
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| - | |frt1-CmR-frt2-T1T2 BBi
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| - | |P17
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| - | |EcoRI
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| - | |PstI
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| - | |
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| - | |frt1-CmR-frt2-T1T2 PCR product digested for cloning as a BB
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| | |} | | |} |
| Digestion Products
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| Number
| Product Name
| Matrix Name
| Enzyme 1
| Enzyme 2
| Size
| Description
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| D22bis
| pSB1A2 BBv
| MP6.1 et .2
| EcoRI
| PstI
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| pSB1A2 vector digested with EcoRI and PstI enzymes
|
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| D88
| pTet>>lox71-gfptripqrt FV
| L34.1 et .2
| EcoRI
| XbaI
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| pTet>>lox71-gfptripqrt pSB1A2 dig. for use as a FV
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| D89
| T-lox66-rbsdapAcoli BV
| L53.7
| SpeI
| PstI
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| T-lox66-rbsdapAcoli pSB1A2 dig. for use as a BV
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| D90
| T-lox66-rbsdapAsubtilis BV
| L56
| SpeI
| PstI
|
| T-lox66-rbsdapAsubtilis pSB1A2 dig. for use as a BV
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|
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| D91
| ptet>>lox71-gfpT-lox66-mrfp pSB1A2 BV
| L59.6, .7
| SpeI
| PstI
|
| ptet>>lox71-gfpT-lox66-mrfp pSB1A2 dig. for use as a BV
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| D92
| rbsdapAcoli BI
| L39.1
| XbaI
| PstI
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| rbsdapAcoli extracted for use as a BI
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| D93
| rbsdapAsubtilis BI
| L64.1, .2
| XbaI
| PstI
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| rbsdapAsubtilis extracted for use as a BI
|
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