BerkiGEM2007-VaibhaviConstructionFile3
From 2007.igem.org
(Difference between revisions)
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Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1145 for the HPI, katG open reading frame from Salmonella typhimurium LT2 | Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1145 for the HPI, katG open reading frame from Salmonella typhimurium LT2 | ||
| - | + | <pre> | |
| - | PCR VU03F.katG /VU06R.katG on HPI, katG (1316 bp, gp = A) | + | |
| - | + | PCR VU03F.katG /VU06R.katG on HPI, katG (1316 bp, gp = A) | |
| + | PCR VU05F.katG/VU04R.katG on HPI, katG (924 bp, gp = B) | ||
--------------------------------------------------- | --------------------------------------------------- | ||
| - | + | PCR VU03F/VU04R on A+B (2212 bp, BglII/XhoI) | |
| - | + | Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L) | |
| - | + | Product is pBca9145-VU2 | |
--------------------------------------------------- | --------------------------------------------------- | ||
VU03F.katG (External oligo) ccaaaAGATCTatgagcacgaccgacgataccc | VU03F.katG (External oligo) ccaaaAGATCTatgagcacgaccgacgataccc | ||
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VU05F.katG (Internal oligo) gccgaaagaagaCctgatctggcag | VU05F.katG (Internal oligo) gccgaaagaagaCctgatctggcag | ||
VU06R.katG (Internal oligo) ctgccagatcagGtcttctttcggc | VU06R.katG (Internal oligo) ctgccagatcagGtcttctttcggc | ||
| + | </pre> | ||
Latest revision as of 22:38, 8 June 2007
Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1145 for the HPI, katG open reading frame from Salmonella typhimurium LT2
PCR VU03F.katG /VU06R.katG on HPI, katG (1316 bp, gp = A) PCR VU05F.katG/VU04R.katG on HPI, katG (924 bp, gp = B) --------------------------------------------------- PCR VU03F/VU04R on A+B (2212 bp, BglII/XhoI) Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L) Product is pBca9145-VU2 --------------------------------------------------- VU03F.katG (External oligo) ccaaaAGATCTatgagcacgaccgacgataccc VU04R.katG (External oligo) gctagCTCGAGttaGGATCCttattgcagatcgaaacggtcc VU05F.katG (Internal oligo) gccgaaagaagaCctgatctggcag VU06R.katG (Internal oligo) ctgccagatcagGtcttctttcggc
