Paris/October 21

From 2007.igem.org

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(Miniprep)
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[[Paris/October 20|yesterday]] -- [[Paris/October 22|tomorrow]] <br>
[[Paris/October 20|yesterday]] -- [[Paris/October 22|tomorrow]] <br>
== Miniprep ==
== Miniprep ==
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The 80mL culture have been treated as a 10mL culture and lysate have been put through 2 different columns. We eluted the DNA in 2*100µL. We finally recovered 2µg of DNA in 200µL for a concentration of 10ng/µL.
== Transformation of Ligation products ==
== Transformation of Ligation products ==

Revision as of 06:14, 22 October 2007

yesterday -- tomorrow

Contents

Miniprep

The 80mL culture have been treated as a 10mL culture and lysate have been put through 2 different columns. We eluted the DNA in 2*100µL. We finally recovered 2µg of DNA in 200µL for a concentration of 10ng/µL.

Transformation of Ligation products

Purification of PCR products

Digestion Reactions