Paris/October 21
From 2007.igem.org
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[[Paris/October 20|yesterday]] -- [[Paris/October 22|tomorrow]] <br> | [[Paris/October 20|yesterday]] -- [[Paris/October 22|tomorrow]] <br> | ||
== Miniprep == | == Miniprep == | ||
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+ | The 80mL culture have been treated as a 10mL culture and lysate have been put through 2 different columns. We eluted the DNA in 2*100µL. We finally recovered 2µg of DNA in 200µL for a concentration of 10ng/µL. | ||
== Transformation of Ligation products == | == Transformation of Ligation products == |
Revision as of 06:14, 22 October 2007
Contents |
Miniprep
The 80mL culture have been treated as a 10mL culture and lysate have been put through 2 different columns. We eluted the DNA in 2*100µL. We finally recovered 2µg of DNA in 200µL for a concentration of 10ng/µL.